VOLKART, P. A.; SPAGIARI, M. S.; BIZANI, D. Estresse e lesões em atletas de esportes coletivos. Arq. Cienc. Saúde UNIPAR, Umuarama, v. 20, n. 1, p, 25-32, jan./abr. 2017. RESUMO:O presente trabalho tem como objetivo estudar o comportamento e a resistência de bactérias em relação a agentes químicos com ação antimicrobiana em diferentes concentrações e determinar a quantidade mínina para que ocorra a inibição dos microrganismos. Serratia marcescens, Proteus mirabilis, Pseudomonas aeruginosas, Escherichia coli, Bacillus cereus e Staphylococcus aureus, foram os microrganismos utilizados, os quais foram semeados em meio Plate Count Agar (PCA). Na sequência, aplicaram-se os testes com os agentes controladores, nas concentrações de 0,5 %, 1,5%, 3,0%, 5,0%, 7,0% e 10,0 %, e os álcoois pré-determinados. A identificação da eficácia foi definida atraves frequência e índice de inibição. Foram realizadas análises de 13 substâncias nas seis diferentes concentrações, ou seja, 468 amostras totalizadas no experimento. Com relação aos microrganismos estudados observou-se, dentre os componentes escolhidos, uma ampla resistência, mesmo em concentrações maiores. O estudo demonstrou variação de resistência e sensibilidade, sendo ácido ascórbico o agente com a maior concentração inibitória mínima e as menores concentrações foram do peróxido de hidrogênio. Considerando importância do uso desses compostos na inibição e no controle de agentes bacterianos em diferentes áreas, torna-se vital o conhecimento da amplitude de seu espectro, assim, alguns produtos, nas diluições recomendadas para uso, revelaram possuir atividade antibacteriana mais reduzida ou inexistente. PALAVRAS-CHAVE: Bactérias. Agentes Químicos. Crescimento Bacteriano. Resistência. BACTERIAL SUSCEPTIBILITY AND RESISTANCE TO GROWTH CONTROLLING AGENTS IN HOSPITAL AND INDUSTRIAL USEABSTRACT: The present work aims at studying the behavior and bacteria resistance against chemical agents with antimicrobial action in different concentrations and determining the minimum quantity for micro-organism inhibition. This study used Serratia marcescens, Proteus mirabilis, Pseudomonas aeruginosa, Escherichia coli, Bacillus cereus and Staphylococcus aureus micro-organisms, which were seeded in Plate Count Agar (PCA) medium. After, the tests were applied with controlling agents, at concentrations of 0.5%, 1.5%, 3.0%, 5.0%, 7.0% and 10.0%, and predetermined alcohols. Effectiveness was identified by frequency and inhibition index. Analyses were carried out on 13 substances in six different concentrations, i.e., a total of 468 samples in the experiment. In relation to the studied micro-organism, a broad resistance could be observed among the chosen components, even in larger concentrations. The study presented resistance and sensitivity variation, with ascorbic acid being the agent with the greatest minimum inhibitory concentration, while hydrogen peroxide presented the lowest concentrations. Considering the importance of these compounds in inhibiting and controlling bacterial agents in differen...

With the growing world development there is a big waste generation by agricultural systems, industrial and besides several accidents with different pollutants, such as petrochemical, metal mechanic, leather and footwear, mining, etc, raising concern with the environment environment and more knowledge about their degradation. In this sense, alternatives are sought to try to minimize environmental problems using living organisms and their enzymes in the biodegradation of toxic compounds aimed at its eradication, reduction or transformation into less toxic substances. To evaluate the growth kinetics, survivability and bioreduction, this study used standard bacterial strains of Acetobacter aceti and Micrococcus luteus in medium containing chromium ion (VI). The chromium ion-containing media were made up by dissolving 850 mg of K2Cr2O7, 1 liter adjusted to the desired concentration of 300 mg L-1. The bioprocess was conducted and 250 ml flask type incubator shaker at 32° C for 7 days. Quantification followed the methodology established by Standard Methods (Method 3500 Cr B). Both strains were shown to be capable of reducing the chromium ion concentrations, containing more than 36% rate. The behavior of strains in liquid medium presented constantly growing and showing no interference of chromium ion.