Aim:To avert the health problems induced by many environmental pollutants, available antioxidants have been evaluated. The present study was aimed to investigate whether α-tocopherol could protect the hexavalent chromium (Cr VI)-induced peroxidation in the liver and kidney and to explore the underlying mechanism of the same.Materials and Methods:A total of 24 Wistar adult female rats were equally divided into four groups. Group 1 served as control while Groups 2 and 3 were administered K2Cr2O7(10 mg/kg b.wt. s.c. single dose). In addition to (Cr VI), Group 3 also received α-tocopherol (125 mg/kg, daily) by oral gavage for 14 days. Group 4 was maintained as α-tocopherol control (dose as above). At the end of 14 days, blood samples were drawn for hematology. Subsequently, all the rats were sacrificed to collect liver and kidney samples for assay of tissue peroxidation markers, antioxidant markers and functional markers and histopathology.Results:Administration of chromium (Cr VI) in Group 2 significantly (P < 0.05) reduced the antioxidant markers such as superoxide dismutase and reduced glutathione along with significant (P < 0.05) increase in peroxidation markers such as malondialdehyde and protein carbonyls in the liver and kidney as compared with other groups. The functional markers in serum such as total protein was decreased significantly (P < 0.05), whereas other functional markers viz. alanine transaminase, blood urea nitrogen and creatinine were increased significantly (P < 0.05) in Group 2 as compared with the other groups. Significant (P < 0.05) decrease in hemoglobin, packed cell volume, total erythrocyte count, mean corpuscular volume, mean corpuscular hemoglobin and total leukocyte count were observed in Cr VI treated Group 2 rats. Prominent pathological changes were observed in the liver and kidney of Group 2. Co-treatment with α-tocopherol in Group 3 rats significantly (P < 0.05) reversed the Cr VI induced changes. The parameters in the study in Group 4 did not differ as compared with Group 1.Conclusions:α–tocopherol exhibited protective effect against Cr VI-induced damage to the liver and kidney by inhibition of lipid peroxidation owing its antioxidant activity.
The present study aimed to investigate the protective effect of ascorbic acid on paraquat induced serum biochemical alterations in experimental rats. Forty-eight (48) male albino Wistarrats were randomized into four (4) groups consisting of twelve (12) in each. Group 1-Control. Group 2 -Paraquat at the rate of 40 mg/kg b.wt. Group 3 -Ascorbic acid at the rate of 250 mg/kg b.wt. Group 4 -Paraquat at the rate of 40 mg/kg b.wt + Ascorbic acid at the rate of 250 mg/kg b.wt. The treatment regimens were administered by oral gavage once daily for twenty-one (21) days. Significantly (P<0.05) increased activity of aspartate transaminase, alanine transaminase, alkaline phosphatase, blood urea nitrogen and serum creatinine and significantly (P<0.05) decreased activity of total protein was observed in group 2 on 7 th and 21 st day of experiment. Cotreatment with ascorbic acid showed a remarkable protection against the parameters investigated possibly via antioxidant defence mechanism.
The therapeutic efficacy of Resveratrol (RSV) and Vitamin E were studied against 5-Flourouracil (5-FU) induced nephrotoxicity. 36 male Wistar rats were selected randomly weighing between 150-180 g and are made into 6 groups, each group containing 6 rats. Group 1 was maintained as sham. 5-Flourouracil was administered to groups 2, 5 and 6 intraperitoneally (20 mg/kg body weight) on day 1, 3 and 7. Group 2 was kept as positive control (administered 5-FU intraperitoneally). Groups 3, 5 and 4, 6 were administered vitamin E and resveratrol per orally for 14 days @ 200 mg/kg bwt. At the end of the experiment the blood was withdrawn and serum analyzed for renal biomarkers. For histopathological studies, samples of kidney tissue collected by inducing euthanasia in rats. The sero-biochemical analysis revealed a significant increase in BUN and creatinine values of the rats in group 2. The antioxidant activity was analyzed and the rats in group 2 revealed a significant rise in the values of protein carbonyl, TBARS and significant decrease in GSH. Group 2 also showed an increase in TNF-α and decrease in interleukin-10 concentration. Sections of kidney tissue collected from group 2 showed marked dilation and elongation of tubules, moderate infiltration with inflammatory cells degeneration of bowman’s capsule and tubular congestion. Comparatively, groups undergone treatment showed amelioration in the parameters. Thus, resveratrol and vitamin-E exert protective actions against 5-flourouracil (5-FU) induced nephrotoxicity.
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