No abstract

We describe a new method for delivering a zinc finger protein directly into cells using a nanoneedle array and an ATP aptamer. The zinc finger protein can be used for site-specific DNA modification by combining with an enzyme that can cut or modify DNA. This controlled release method utilizes aptamer conformational changes to deliver various biomolecules specifically into cells.

No abstract

Genome editing with site-specific nucleases (SSNs) may be effective for gene therapy, as SSNs can modify target genes. However, the main limitation of genome editing for clinical use is off-target effects by excess amounts of SSNs within cells. Therefore, a controlled delivery system for SSNs is necessary. Previously we have reported on a zinc finger nuclease (ZFN) delivery system, which combined DNA aptamers against FokI nuclease domain (FokI) and nanoneedles. Here, we describe how DNA aptamers against FokI were selected and characterized for genome editing applications.