This study aimed to assess the effects of light regime and time of slaughter on primal cut and organ weights, peptide transporter 1 (PEPT1) gene expression in the jejunum, arylalkylamine N-acetyltransferase (AANAT) gene expression in the brain, and liver oxidant/antioxidant status in broilers aged 37 days. The experiment was conducted in a factorial completely randomized design, with two light regimes (intermittent light varying according to bird age and continuous light under an 18 h light/6 h dark photoperiod) and four times of slaughter (2:00, 8:00, 14:00 and 20:00 h). There was an interaction effect on PEPT1 and AANAT expression, lipid and protein oxidation and superoxide dismutase (SOD) activity. In both light regimes, PEPT1 expression responded cubically to slaughter time. In the continuous light group, PEPT1 expression was highest in birds slaughtered at 2:00 and 14:00 h, whereas, in the intermittent light treatment, expression was highest at 8:00 h. In the continuous light regime, AANAT expression had a cubic relationship with time of slaughter, with the greatest values recorded at 20:00 h. In the intermittent light regime, slaughter time showed a cubic effect on lipid oxidation, which was highest at 8:00 h. In the continuous light group, there was a cubic effect on nitrite concentration, lipid oxidation, protein oxidation, and SOD activity; nitrite levels, lipid oxidation, and protein oxidation were highest and SOD activity was lowest in birds slaughtered at 14:00 h. Time of slaughter influenced catalase activity, which responded cubically; catalase activity was lowest at 8:00 and 14:00 h. This study is the first to demonstrate that PEPT1 expression in the jejunum of broilers follows a diurnal rhythm and varies according to light regime. The results also suggest that mainly continuous lighting and slaughter at 14:00 h when the animals are possibly more active may be more stressful to broilers.

O mosquito Aedes aegypti (conhecido como mosquito da dengue ou pernilongo rajado), vem se espalhando em quase todo o planeta, principalmente nas regiões tropicais e subtropicais. O Aedes aegypti é o principal vetor de múltiplas doenças, como dengue, zika e chikungunya. Uma das estratégias em muitos municípios brasileiros na tentativa de combater esse mosquito é a utilização do ‘Fumacê’, um defensivo líquido que tem na sua principal composição Malathion em Emulsão Aquosa (EA) 44%. O presente estudo teve como objetivos identificar quais os efeitos negativos para seres vivos e meio ambiente devido ao uso inadequado do Malathion no combate ao mosquito da dengue. Foi realizado um levantamento nas bases de dados (PUBMED, Science Direct e Google Scholar), nos últimos cinco anos (2014-2018), usando as restrições ou combinações de termos: ‘Malathion’, ‘Malation’, ‘Malatião’, ‘Aedes aegypti’, ‘A. aegypti’, ‘Mosquito da Dengue’, ‘Inseticida’, ‘Organofosforado’, ‘Animais Nocaute’, ‘Intoxicação’. A pesquisa procurou identificar dados sobre riscos do uso inadequado do Malathion para a saúde humana, a toxicidade do defensivo, o impacto sobre o meio-ambiente, as alternativas de biorremediação e algumas opções biotecnológicas. Desta forma, o defensivo Malathion através de estudos in vivo em animais como: Cyprinus carpio (carpas); Rattus norvegicus (ratos Wistar) e testes in vitro com células de linhagens humanas mostrou efeitos tóxicos em baixas concentrações. Estes dados revelam a necessidade de mais estudos sobre este defensivo para melhor entendimento de seus efeitos sobre os seres vivos e o meio ambiente.