No abstract

Extracts of human gastric mucosa have been fractionated on diethylaminoethyl cellulose to yield three precursors of proteolytic enzymes active at low pH which have been called pepsinogen I, pepsinogen II, and pepsinogen III. Pepsinogen I has been found in the mucosa from all parts of the stomach examined as well as in the proximal duodenum. Pepsinogen II and pepsinogen III were present in fundic mucosa only.

Extracts of acidified human gastric fundic mucosa have been fractionated on diethylaminoethyl cellulose to yield three pepsin fractions, which have, in order of their elution, been attributed to pepsin I, pepsin II A, and a mixture of pepsin II B and pepsin III. Acidification of previously purified pepsinogen I and pepsinogen III yields pepsin I and pepsin III, respectively. Acidification of previously purified pepsinogen II yields two distinct pepsin fractions, pepsin II A and pepsin II B. Pepsin II B and pepsin III have the same chromatographic mobility on diethylaminoethyl cellulose, but are probably not identical. Pyloric and duodenal mucosa yield only pepsin I upon acidification. Human pepsin-pepsin inhibitor complex is demonstrable as a chromatographic fraction distinct from pepsins upon fractionation of acidified dilute pepsinogen solutions with diethylaminoethyl cellulose equilibrated with acetate buffer pH 5.3.

Acid gastric juices have been fractionated on DEAE (diethylaminoethyl)-cellulose to yield three pepsin fractions, which have the same chromatographic mobility as the three pepsin fractions previously described in acidified whole gastric mucosal extracts. The acid gastric juices included specimens obtained from healthy individuals and from patients with peptic ulcer and gastritis. Gastric juice from one patient with pernicious anemia was shown to contain only pepsinogen I. A satisfactory chromatographic separation of pepsins is possible from as little as 4 ml of gastric juice.

Page 1099: Max J. Seijffers, Harry L. Segal, and Leon L. Miller. "Separation of pepsin I, pepsin II A, pepsin II B, and pepsin III from gastric mucosa." Title in abstract, in issue table of contents, and in volume table of contents printed in error as title of companion paper which begins on page 1106. Should read the same as above title.