Separation of pepsin I, pepsin II A, pepsin II B, and pepsin III from human gastric mucosa

Abstract

Page 1099: Max J. Seijffers, Harry L. Segal, and Leon L. Miller. "Separation of pepsin I, pepsin II A, pepsin II B, and pepsin III from gastric mucosa." Title in abstract, in issue table of contents, and in volume table of contents printed in error as title of companion paper which begins on page 1106. Should read the same as above title.

Pepsinogen

“…Ca + removal partly or completely inhibits most stimuli (Koelz et al, 1982;Inoue et al, 1983), and dibutyryl cAMP stimulates in all preparations. All stimuli increased cAMP production in the presence of the phosphodiesterase inhibitor IBMX (Shirakawa et al, 1983;Inoue et al, 1983). The combined evidence suggests that both Ca++ and cAMP are involved in stimulation by all active stimuli-CCK in rabbit and guinea-pig, cholinergic and adrenergic in mammals and the frog, and bombesin in the frog.…”

“…Cholinergic stimulation is reported for all preparations (Koelz et al, 1982;Hersey et al, 1983;Simpson, Goldenberg and Hirschowitz, 1980) as is isoproterenol (Koelz et al, 1982;Hersey et al, 1983;Shirakawa et al, 1983), a weaker stimulus. Both are blocked by their specific antagonists, atropine (Inoue et al, 1983) and propanolol (Shirakawa et al, 1983). In the frog, bombesin is also a potent stimulus of pepsinogen secretion (Shirakawa et al, 1983).…”

“…Pepsinogen can be electrophoretically separated into multiple bands representing decreasing electronegativity or anodal mobility (Richmond et al, 1958;Seijffers, Segal and Miller, 1963;Kushner, Rapp and Burtin, 1964;Samloff, 1969). There appear to be about seven pepsinogens (Pg 1-7) that conform to the operational definition of inactive precursors resistant to alkaline pH, but which, upon acidification, convert to acid active proteases with pH optima near 2-0 and susceptibility to inactivation above pH 7-2.…”

“…Both are blocked by their specific antagonists, atropine (Inoue et al, 1983) and propanolol (Shirakawa et al, 1983). In the frog, bombesin is also a potent stimulus of pepsinogen secretion (Shirakawa et al, 1983). Ca + removal partly or completely inhibits most stimuli (Koelz et al, 1982;Inoue et al, 1983), and dibutyryl cAMP stimulates in all preparations.…”

“…In the frog, bombesin is also a potent stimulus of pepsinogen secretion (Shirakawa et al, 1983). Ca + removal partly or completely inhibits most stimuli (Koelz et al, 1982;Inoue et al, 1983), and dibutyryl cAMP stimulates in all preparations. All stimuli increased cAMP production in the presence of the phosphodiesterase inhibitor IBMX (Shirakawa et al, 1983;Inoue et al, 1983).…”