Abstrak Jeruk purut (Citrus hystrix DC.) merupakan salah satu keanekaragaman flora di Indonesia. Jeruk purut oleh masyarakat Indonesia dimanfaatkan sebagai penyedap masakan dan aroma terapi. Penelitian mengenai jeruk purut masih terbatas dilakukan dibandingkan ketersediaan jeruk purut di alam yang melimpah. Penelitian ini bertujuan untuk mengetahui kandungan metabolit sekunder ekstrak etanol daun jeruk purut yang terdiri dari flavonoid, alkaloid, polifenolat, kuinon, monoterpenoid dan seskuiterpenoid. Penelitian ini dilakukan dengan metode ekstraksi kemudian dianalisis penapisan fitokimia simplisia daun jeruk purut secara kualitatif. Hasil penelitian menunjukkan ekstrak daun jeruk purut positif mengandung senyawa alkaloid, flavonoid, polifenolat, kuinon, monoterpenoid dan seskuiterpenoid. Kata kunci : Daun citrus hystrix, ekstraksi, metabolit sekunder Abstract Lime (Citrus hystrix DC) is one of the flora diversity in Indonesia. Lime has been used as a seasoning and aromatherapy by Indonesian people. Research of lime still limited compared to lime availability which is very rich in the nature. The purpose of the research is to discover the content of ethanol extract of lime leaves which is consist of flavonoids, alkaloids, poliphenolics, quinone, monoterpenoin, and sesquiterpenoin. The research is conducted with extraction method then the phytochemical screebning of lime leaves simplicia has been analyzed with qualitative methode. The result show that lime leeaves extract positively contain alkaloids, flavonoids, polyphenolics, quinone, monoterpenoid and sesquiterpenoid compound. Keywords: Citrus hystrix leaves, extraction, secondary metabolite

Diarrhea can occur due to food and beverage poisoning, with the highest cause being caused by infection with various bacteria, viruses, or parasites. Bacteria that can cause this disease are Escherichia coli bacteria which are known as good bacteria in the digestive tract. But the reality is that in microbiology not all types of Escherichia coli are good bacteria. Aim to find out the content and number of Escherichia coli bacteria colonies in UHT milk brands A and B as well as brand x yogurt products. Identification of Escherichia coli bacteria by the IMVIC Test method (Indole, Methyl-Red (MR) test, Voges Proskauer (VP), and Citrate), TPC (Total Plate Count), bacterial staining and microscope observation. Negative and positive results were obtained in the indole test and the methyl-red test was characterized by the formation of a red ring at the top for positive results and a yellow ring at the top for negative results, as well as negative results obtained in the Voges Proskauer test and the citrate test. Then for the results of gram staining and microscope testing the bacterial morphology was not seen. For the calculation of colonies, 45 colonies of sample A, 60 colonies of sample B, and 38 colonies of sample X. Samples containing Escherichia coli are contained in sample A of UHT milk and sample X of yogurt products and microbial contamination in samples according to SNI 2009.