The present investigations involving forty five diverse pea recombinant inbreds and three standard checks were carried out for two successive years during 2011-2012 and 2012-13. The objective of the study was to ascertain genetic variability, heritability, genetic advance, correlation and path coefficient among yield and other horticultural and quality traits and identification of superior recombinants for their utilization in crop improvement programme. Wide range of variability was observed for most of the characters under study. The magnitude of phenotypic coefficient of variation (PCV) was higher than the genotypic coefficient of variability (GCV) for all the traits. High phenotypic and genotypic coefficient of variations were recorded for protein content, ascorbic acid, plant height and pod yield per plant for both the years. Pod yield per plant and plant height exhibited high heritability associated with high genetic advance in the respective years indicating the additive gene action for their expression and are likely to respond better to selection. Pod yield showed a positive and significant correlation with number of pods per plant, pod length and number of seeds per pod both the years. Path analysis revealed that number of pods per plant and pod length have highest positive direct effect both the years and thus it may be treated as selection criteria for isolating higher yielding genotypes in garden peas. In heterosis study, some of the recombinant inbreds were found superior over standard checks which include L-14 and L-13 for pod yield/plant, number of pod/plant and earliness. These findings can be further utilized to develop and enhance the yield potential of garden pea.
The present investigation was carried out using 51 diverse bitter gourd accessions as material for studying genetic diversity and relatedness using morphological and SSR markers. A wide variation was observed for morphological traits like the number of days to the first female flower anthesis (37.33–60.67), the number of days to the first fruit harvest (47.67–72.00), the number of fruits/plant (12.00–46.67), fruit length (5.00–22.23 cm), fruit diameter (1.05–6.38 cm), average fruit weight (20.71–77.67 g) and yield per plant (513.3–1976 g). Cluster analysis for 10 quantitative traits grouped the 51 accessions into 6 clusters. Out of 61 SSR primers screened, 30 were polymorphic and highly informative as a means to differentiate these accessions. Based on genotyping, a high level of genetic diversity was observed, with a total of 99 alleles. The polymorphic information content (PIC) values ranged from 0.038 for marker BG_SSR-8 to 0.721 for S-24, with an average of 0.429. The numbers of alleles ranged from 2 to 5, with an average of 3.3 alleles per locus. Gene diversity ranged from 0.04 for BG_SSR-8 to 0.76 for S-24, showing a wide variation among 51 accessions. The UPGMA cluster analysis grouped these accessions into 3 major clusters. Cluster I comprised 4 small, fruited accessions that are commercially cultivated in central and eastern India. Cluster II comprised 35 medium- to long-sized fruited accessions, which made up an abundant and diverse group. Cluster III comprised 11 long and extra-long fruited accessions. The polymorphic SSR markers of the study will be highly useful in genetic fingerprinting and mapping, and for association analysis in Momordica regarding several economic traits.
Abstract:The present investigation involves 29 diverse bell pepper genotypes for variability studies and 14 promising lines (11 lines and 3 testers), 33 F 1 and 1 standard check for combining ability studies. The objective of the study was to assess the nature and magnitude of genetic variability among the bell pepper genotypes for yield and other horticultural traits and to identify the potential parental lines and cross combinations suitable for the protected environment based on the estimation of general combining ability and specific combining ability. A wide range of variability was observed for most of the characters studied. The genotypes viz., Kashmir Sel-1 (L 2 ), DARL-10 (L 9 ) and UHFSP(Y)-11 (L 11 ) for marketable fruit yield per plant and Kashmir Sel-1 (L 2 ), UHFSP(Y)-11 (L 11 ) and DARL-01 (L 5 ) for earliness were good general combiners and these may be included for exploiting hybrid vigour or development of purelines. On the basis of specific combining ability effects and per se performance, the crosses viz.,
Aroids are an important group of indigenous tuber crops, grown widely for their leaves, petioles, stolons, corms, and cormels. A total of 53 genotypes were evaluated for their genetic diversity in northeastern region of India. At household level, a total of 16 landraces of Aroids were recorded having different ethnobotanical uses. Based on the population study under Jhum/Shifting farming, landrace Rengama was dominant in area with 47% of the total population followed by Tamachongkham and Tasakrek. However, Pugarkusu and Chigi occupied 33.0 and 24.0% of the population, respectively under backyard farming, and were considered as major landraces. Tamachongkham, high in acridity and total oxalate content (0.82%), was used for cooking with meat, while Tasakrek was used as a baby food due to high total sugar (>3.0%), low in acridity, and total oxalate content (<0.12%). The Simpson’s diversity index of the backyards was higher (0.80) as compared to Jhum field (0.63). The genotypes showed wider variability in growth and yield attributes like; plant height (89.4–206.1 cm), number of side shoots (1.84–5.92), corm weight (38.0–683.3 g), cormel weight (14.0–348.3 g), yield (0.24–1.83 kg plant−1). Similarly, wide variations were also observed for quality traits like total sugar (1.93–4.94%); starch (15.32–32.49%), total oxalate (0.10–0.82%), and dry matter (16.75–27.08%) content. Except for total oxalate, all the growth and yield attributes have shown high heritability and moderate to high genetic advance. Molecular analysis (33 polymorphic SSR markers) detected a total of 136 alleles, ranged 3 to 8 alleles per marker. The observed heterozygosity (0.24) was less than expected heterozygosity (0.69). The group-wise maximum genetic divergence was observed between Colocasia fallax (cv. Chigi) to C. esculenta var. aquatilis (cv. Tharsing); C. fallax (cv. Chigi) to C. gigantea (cv. Ganima) and C. gigantea (cv. Ganima) to Xanthosoma spp., while it was least between eddo and dasheen. The findings indicated, a wider diversity and distinct ethnobotanical uses of Aroid landraces at the house hold levels, which should be conserved and popularized to ensure nutritional security.
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