The generally accepted concept that metabolic acidosis in infant diarrhea results from the loss of buffer base in the stool has been based on the common observation that inorganic cations predominate over inorganic anions in diarrheal stools (1)(2)(3)(4)(5). This predominant loss of inorganic cations has been taken as evidence of loss of bicarbonate. On the other hand, Teree, Mirabal-Font, Ortiz, and Wallace (6) have shown by direct measurements that the pH of the stool water in infant diarrhea is frequently acidic (almost always below the pH of the blood) and that HCO3-is very low or absent. On the basis of the observed differences between Na+ + Ki and Cl-, these authors have suggested that there is a high concentration of organic anions in diarrheal stools of infants and have advanced several hypotheses to explain the origin of these substances (7).The effect of fecal loss of organic anion on acidbase balance will depend upon several factors. These include the origin of the organic anions (i.e., whether ingested preformed, or generated within the body) and the nature of, the cations accompanying them (8). Those organic acids generated in the intestinal lumen or elsewhere in the body change the H+ concentration of the body fluids, depending upon the degree of ionization of
The present study aimed to investigate, in the streptozotocin-induced mild diabetic rat model, the zinc (Zn), copper (Cu), iron (Fe), calcium (Ca), and magnesium (Mg) concentration in serum, liver, and kidney tissues, and urine samples from adult Wistar rats treated neonatally with streptozotocin (STZ). Diabetes was induced by subcutaneous administration of streptozotocin (100 mg/Kg) in female Wistar rats of 2 days old (STZ, n = 10). Control group (CG, n = 10) received only sodium-citrate buffer. The mineral concentrations were measured by atomic absorption spectrophotometry. The validity and accuracy were checked by conventional methods. STZ neonatal injection successfully leaded to mild diabetes in the adult rats. Serum concentrations of Zn, Cu, Fe, Ca, and Mg showed no changes (p > 0.05) due to diabetes. The Zn, Fe, Ca, and Mg concentrations in liver and kidney tissues were not different (p > 0.05) between STZ and CG. The mean values of Cu were higher (p < 0.05) in liver and kidney samples from STZ as compared to CG. Urine minerals concentrations (Zn, Cu, Fe and Ca) in STZ-rats group were lower (p < 0.05) than CG. However, the content of all evaluated minerals in the excreted urine were higher (p < 0.01) in STZ-rats during a 24 h collection period. Urinary excretion of Zn, Cu, Fe, Ca, and Mg was strongly correlated with urinary volume during the 24 h period (r > 0.7; p < 0.001). Observed changes in mineral metabolism of STZ-induced mild diabetes model could be due to the endocrine imbalance associated with the diabetic condition.
scite is a Brooklyn-based organization that helps researchers better discover and understand research articles through Smart Citations–citations that display the context of the citation and describe whether the article provides supporting or contrasting evidence. scite is used by students and researchers from around the world and is funded in part by the National Science Foundation and the National Institute on Drug Abuse of the National Institutes of Health.
customersupport@researchsolutions.com
10624 S. Eastern Ave., Ste. A-614
Henderson, NV 89052, USA
This site is protected by reCAPTCHA and the Google Privacy Policy and Terms of Service apply.
Copyright © 2024 scite LLC. All rights reserved.
Made with 💙 for researchers
Part of the Research Solutions Family.