A study aimed to examine immunomodulatory effect of concentrated cell free filtrate (CCF) of Lactobacillus acidophilus on immune system of mice by detecting the total and absolute counts of leucocytes. It was shown that CCF of Lactobacillus acidophilus caused a significant increase in total leucocyte count for all concentrations used in comparison with negative control. However, in absolute leucocyte counts the, CCF of L.acidophilus at doses (500 , 750) mg/kg caused a significant increased in lymphocyte, neutrophil and monocyte counts, while there was no significant differences in eosinophils and basophil cells number of mice treated with bacterial filtrate. The interaction study showed that the post-treatment with CCF of L. acidophilus caused a significant increase in total leucocytes count and absolute counts of all types of leucocyte cells except basophils and esinophils which showed no significant differences in comparison with pre-treatment

This study dealt with the effect of Lactobacillus acidophilus concentrated filtrate on polychromatic erythrocyte. The result showed that Lactobacillus acidophilus concentrated filtrate caused a non significant decrease in micronucleus formation at first dose in comparison with negative control while a significant decrease in micronucleus formation was shown in albino male mice at second and third doses in comparison with negative and positive controls. The result of post-treatment by concentrated filtrate also caused a significant decrease in micronucleus formation while the pre-treatment caused non significant decrease in the micronucleus formation in comparison with the corresponding control.

Twenty swab samples were taken from patients suffering from otitis infection; these samples were cultured on different agar media (blood agar base, macConky and cetramide). Several microscopical, morphological and biochemical tests were performed and results showed that 50% of isolated colony were belonged to Psudomonas aeruginosa. In addition it was found that males were more susceptible to infection with otitis than female especially at teenager aged. Five isolates were subject to sensitivity test against several antibiotic groups. Result showed that all five isolates were resistance to pencilline, tetracycline, Cefotaxtin (100%) and (60%) of them showed resistance to cefotaxime and (40%) to chloroemphenicol and (20%) to amoxicillin while all isolates were sensitive to ciprofloxacin. The minimum inhibitory concentration (MIC) test was performed for two multiple drugs resistance isolates (P1 and P2) result declared that both P1and P2 showed high-level of resistance to Cefotaxime and chloraemphenicol with MIC (128μg\ml), followed by (64μg\ml) to ampicillin while P2 was (128 μg\ml), as well as, the amoxicillin showed the lowest MIC (16 μg\ml) forP1 and (64 μg\ml) to P2.No pH effect was observed on the activity of garlic when antibacterial effect of garlic extract against Pseudomonas aeruginosa was investigated at different PH. The MIC of garlic extract on Pseudomonas aeruginosa at different incubation periods was determined. Results showed that after 24hrs MIC of extract was greater than 8μg/ml for P1 isolate and 16μg/ml for P2 isolate.While, after 72rs it was found that the MIC at concentrations (32, 64 and 128 μg/ml) were more effective in inhibition growth of (P1, P2) while in (8 μg/ml) that indicated heavy growth of P. aeruginosa