Lack of clean water supply, sanitation and hygiene are major causes for the spread of waterborne diseases in a community. This study was conducted, to determine the quality of drinking water collected from Kosti City, White Nile State, Sudan. In this study, the quality levels of drinking water (physical, chemical and the microbial), were determined following the standard methods. Five sites were selected to sampling drinking water during Feb. 2021. The results showed that, some of pH, HCO3-, Hardness, SO-, Na+ and Ca++ values were not fall within the standard ranges. Total Dissolved Solids (TDS), Cl and K+ data agreed with standard ranges. CO3 show a very strong +ve correlation (0.99) with Na and -ve correlation (-0.98) with HCO3. E. coli were relatively high in comparison to standards (which should be zero). The study recommends evaluating the quality of the drinking water, and this should be routine work.