A cross-sectional study was conducted in the Department of Microbiology at tertiary care hospital in Bangladesh from July 2019 to June 2020, to see the phenotypic and genotypic characterization of Salmonella Typhi isolated from human blood with distribution of virulence genes and sequencing. Clinically suspected enteric fever patients irrespective of age and gender who attended either the Department of Microbiology or Medicine out-patient department (MOPD) in a tertiary level hospital in Bangladesh for blood culture and sensitivity test were included in this study. The positive growth S. Typhi was identified by gram straining, colony morphology and biochemical test. Then Salmonella Typhi was identified by using Salmonella specific antisera. Final identification was done by using 16s Rrna by PCR. Virulence factors like hemolysin and siderophore was detected by siderophores production assay. PCR and sequencing of virulence genes were done. A total number of 50 isolated organisms were identified as Salmonella species of which Salmonella Typhi was detected in 40 (48.2%) isolates. Among 40 isolated Salmonella Typhi, no Salmonella Typhi produced hemolysin and 30 (75.0%) isolates have ability to produce siderophore. Among the isolated Salmonella Typhi all isolates 40 (100%) were positive for viaB and staA gene, 21 (52.5%) were positive for iroB. Sequencing of viaB and iroB were done for further validation. In conclusion all isolated Salmonella Typhi carried viaB and staA, iroB genes were responsible for S. Typhi pathogenicity.
CBMJ 2023 January: Vol. 12 No. 01 P: 41-47
The current study was conducted to evaluate the socio-economic profile of fisher communities in Dengar beel under Melandah Upazila of Jamalpur district, Bangladesh. A total of 45 fishers were surveyed from November 2020 to April 2021. It was found that the family size of 53% of fishers was medium, consisting of 5-6 members. Most of the fisher communities belonged to the age group above 45 years old, represented by 100% Muslim. About 73% of fishers were less educated, 13% primary level, 7% secondary level, and 7% Secondary School Certificate passed and above, respectively. Most of the fishers (60%) have katcha houses with tin roofing, 7% have katcha house with straw roofs, 13% have semi- pucca houses and 20% have pucca houses. Around 93% of fishers used their own tube-well while only 7% used neighbours’ tube-well. All of the fisher household were connected with electricity supply. About 27% of fisher communities used katcha toilets, 46% used semi pucca toilets and 27% used pucca toilets. About 47% of fisher communities were dependent on village doctors, 33% got health service from Upazila health complex, 20% from MBBS doctors, and others. About 46% of fishers were engaged in fishing as their primary occupation while 27% in agriculture with fishing, 20% in daily labour with fishing and 7% in livestock rearing. About 33% of the fishers had low income, 40% had medium income and 27% had high income. The present study revealed that the fishers of Dengar Beel were mostly less educated, less privilege to attain modern technology-oriented training experience, less income and less awareness about sanitation and health facilities. So, provide soft loans to fishers, and strengthening people awareness has been might be helpful to improve the socio-economic status of fisher communities in Dengar Beel.
Asian J. Med. Biol. Res. 2021, 7 (2), 164-173
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