Curing preservation of fish is the most popular method in the developing countries and India is no exception. Different film‐forming gums, namely sodium alginate, tamarind kernel powder, guar gum and agar agar were tried to find their effect in preservation of salted and dried mackerel and in extending the shelf‐life of these products. Various biochemical, microbiological and organoleptic parameters were recorded during the storage period. The treatment of salted dried fish with gum solutions and subsequent formation of film upon drying was found to be the best in preserving the quality of salted fish. The effect of film forming gums on the shelf‐life of the products was found to be highly significant (P<0·05) as compared to the control.

Background: Stem cells from human exfoliated deciduous teeth (SHED) are regarded as an attractive cell source for tissue regeneration. However, the effect of different levels of root resorption on the characteristics of SHED remains less understood. Thus, the tooth source that is most suitable for the isolation of SHEDs needs to be determined. To compare cellular and biological characteristics of stem cells from human exfoliated deciduous posterior teeth with varying levels of root resorption. Methods:The pulp was obtained from the deciduous posterior teeth depending on the level of root resorption, and isolated SHEDs were grouped as follows: Teeth with 0 to 1/3 rd root resorption as SHEDs (G1) and 1/3 rd to 2/3 rd root resorption as SHEDs (G2). Teeth were also collected from >2/3 rd root resorption status, but failed to establish primary culture of SHED as the availability of pulp tissue was too less. Later, isolated SHEDs were compared on their morphology, viability, growth kinetics, colony-forming ability, expression of cell surface markers and in vitro differentiation into osteocytes and adipocytes.Results: No major differences were observed in terms of cellular morphology, viability, proliferation rate, colony-forming ability, cell surface markers expression, and mesenchymal lineage differentiation of SHEDs isolated from posterior teeth with 0 to 1/3 rd and 1/3 rd to 2/3 rd root resorption. However, SHED from teeth with 0 to 1/3 rd root resorption (G1) displayed relatively higher proliferation capacity and expression of selected markers.Conclusions: Collectively, SHEDs (G1) and SHEDs (G2) showed comparable cellular and biological characteristics that enable their possible applications in regenerative therapies.

Background: Deciduous teeth undergo the physiologic process of resorption, during which the remnant pulp undergoes activation. However, the quality of stem cells obtained at various stages of root resorption has not been documented. Objective: To isolate and characterize stem cells from deciduous teeth with varying levels of root resorption. Study design: Healthy primary anterior teeth were extracted according to the treatment needs of the patient. The teeth were categorized into SHED(1/3)- teeth with 0 to 1/3rd root resorption, SHED(2/3)- teeth with 1/3rd to 2/3rd root resorption, and SHED(COMP)- teeth with more than 2/3rd root resorption. SHED were characterized based on their morphology, viability, proliferation rate, population doubling time, expression of cell surface markers, and in vitro differentiation potential into osteocytes and adipocytes. Results: SHED from all three groups demonstrated largely similar morphological and cellular characteristics. However, SHED(2/3) showed relatively better characteristics in terms of growth kinetics and phenotypic marker expression. Also, the differentiation ability for osteogenic and adipogenic cell lineages was slightly higher in SHED(1/3) and SHED(2/3) compared with SHED(COMP). Conclusion: Based on the cellular, phenotypic and biological characteristics, it is suggested that SHED (2/3) could be a useful source for tissue regeneration, and warrants further investigations.

A bstract Tooth loss due to trauma often triggers residual alveolar resorption to a greater degree in the sagittal direction leading to atrophy. However, in a pediatric patient, if this defect is left untreated, it can cause further atrophy leading to collapse of the arch. In the maxillary anterior area, this is also of esthetic concern. Hence, it is viable to reconstruct the alveolar defects and restore the alveolar anatomy with superior quality of bone while the patient is still in growing phase to avoid any structural and dental malformation, as well as to provide a more novel treatment like dental implant at a later stage when growth ceases. Modes for successfully augmenting the bone are diverse. Among these, barrier membrane with guided bone regeneration, distraction osteogenesis, and bone block graft is ubiquitous. The current case report deals with the management of horizontal atrophic anterior maxillary region using autologous block bone graft harvested from mandibular symphysis, to augment the alveolar ridge and aid in esthetic and functional restoration of alveolar anatomy by restoring the defect with the bone of superior quality as well as preparing the site for receiving implant prosthesis in future when growth ceases. How to cite this article: Bhandary M, Hegde AM, Shetty R, et al. Augmentation of Narrow Anterior Alveolar Ridge Using Autogenous Block Onlay Graft in a Pediatric Patient: A Case Report. Int J Clin Pediatr Dent 2021;14(2):311–314.

In the present investigation, fish cutlet was prepared from recovered meat of filleting residue of Epinephelus chlrostigma and their effect on the biochemical and sensory characteristics have been studied during the frozen storage. Biochemical parameters viz., total volatile base nitrogen, trimethylamine nitrogen, peroxide value, free fatty acids and thiobarbituric acid value of prepared fish cutlet were found to increase during the frozen storage (−20C) of 4 months significantly (P < 0.05) and the increment was within the prescribed limits. Sensory analysis reveals a decreasing trend in the attributes like appearance, color, taste, texture, flavor and the overall acceptability was judged to be good at the end of storage study. This study concluded that the meat can be recovered from filleting waste and suitable for the preparation of ready-to-serve fish cutlet without any significant changes in biochemical and sensory evaluation during frozen storage. PRACTICAL APPLICATIONSThe present study pave the way for maximum and better utilization of filleting waste of reef cod and also describe the quality changes of prepared fish cutlet during frozen storage. Recovery of meat from fish processing waste and product development add the economic value to the seafood industry and also alleviate the pollution and legal problems associated with the discard of processing waste.