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Abstract. Lipid-soluble antioxidants can be more effective for chick development when provided via in ovo (IO) injection than when supplemented to the maternal diet. This study was conducted to evaluate the effects of egg weight (EW) and IO injection of α-tocopherol on chick development, hatching performance and lipid-soluble antioxidant concentrations in residual yolk sac (RYS), liver and brain tissues of quail chicks. Eggs were obtained from quail breeders at days 72 and 128 of age and incubated at 37.8 ∘C and 60 % relative humidity. Each egg was numbered and weighed prior to incubation, and the average EW of all eggs was 11.76 ± 0.05 g. The eggs were divided into light (< 11.76 EW; EWL) and heavy (> 11.76 EW; EWH) groups (148 eggs per EW). Each EW group was divided into two IO groups: the control (non-injection) group and α-tocopherol group, in which 3.75 mg of α-tocopherol per egg was injected into the yolk sac followed by a 120 h incubation period. There were 64 eggs for each EW–IO treatment combination (16 eggs per EW per tray). The chick and RYS weights were significantly lower in the EWL group than in the EWH group. A significant EW by IO interaction suggested that IO increased the eggshell temperature of light quail eggs. The non-injected light eggs had a shorter hatching time due to the interaction of EW with IO. Hatchability, embryonic mortalities, and the cumulative hatching rate were not affected by EW or IO. The chick and middle toe lengths increased following IO administration (P < 0.05), which indicated that IO administration had positive effects on chick quality. However, shank length decreased following IO administration with an unchanged relative asymmetry (RA). IO significantly affected the absolute weights of the liver and heart and the relative weight of the heart, which was lower in the α-tocopherol group than in the control group. IO administration had no effect on total retinol and carotenoid concentrations in the RYS, liver and brain. Vitamin E, α-tocopherol and δ-tocopherol concentrations in the RYS, liver and brain were significantly higher in the α-tocopherol-treated chicks than in the control chicks. The highest brain α-tocopherol concentration was found in the α-tocopherol-treated chicks of the EWH group, indicating a significant interaction between EW and IO. The highest total lipid-soluble antioxidant concentrations were obtained following IO α-tocopherol treatment (P < 0.05), in the order brain < liver < RYS, but this pattern was not observed with δ-tocopherol. In conclusion, IO injection of α-tocopherol into the yolk sac affected the concentrations of tissue-specific lipid-soluble antioxidants in the RYS and tissues of newly hatched quail chicks, and EW had effects on various parameters.

The use of natural antioxidants, in particular polyphenols such as dihydroquercetin (DHQ), in animal nutrition have recently increased in popularity. This may partly be due to the risk of increased incidences of heat stress associated with raising livestock in warmer ambient temperatures, facilitated by global warming, reducing antioxidant capacity. The current research demonstrates the effect of dietary DHQ, vitamin E and standard or high ambient temperatures on growth performance, energy and nutrient metabolism, gastrointestinal tract development (GIT), jejunal villus morphometry and antioxidant status in broiler chickens.Each of the four experimental diets were fed to 16 pens of five birds, which were allocated to four rooms (four pens in each room). The temperature in two rooms was maintained at a constant 35 °C (high temperature; HT), and the temperature in the other two rooms was gradually reduced from 27 °C at 7d of age to 22 °C at 20d of age (standard temperature; ST).Rearing birds at HT reduced: feed intake, weight gain, weight of small intestine, total GIT, liver, spleen, heart, villus height, villus surface area and lowered blood glutationperoxidase (GSH-Px). Dietary DHQ increased blood GSH-Px and total antioxidant status, increased heart weight and reduced caecal size. When fed separately, DHQ and vitamin E improved hepatic vitamin E concentration. Feeding vitamin E increased spleen and liver weights. When fed together, DHQ and vitamin E reduced villus height, villus height to crypt depth ratio and villus surface area. Temperature and antioxidants did not affect energy and nutrient metabolism. There were no effects of dietary antioxidants on growth performance of broiler chickens and there were no mortalities. At present it is unclear if feeding antioxidants (in particular DHQ) at different levels, using different dietary formulations, and rearing birds under a range of environmental conditions may be effective at enhancing production performance and bird health in hot ambient climates.

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