The expression of the genes for the major outer membrane proteins OmpF and OmpC are osmoregulated. The ompC locus was found to be transcribed bidirectionally under conditions of high osmolarity and a 174-base transcript encoded upstream of ompC was found to inhibit the OmpF production and to substantially reduce the amount of the ompF mRNA. This RNA [mRNA-interfering complementary RNA (micRNA)] has a long sequence that is complementary to the 5' end region of the ompF mRNA. We propose that the micRNA inhibits the translation of the ompF mRNA by hybridizing with it. This RNA interaction may cause premature termination of the transcription of the ompF gene or destabilization of the ompF mRNA or both.

No abstract

DNA polymerase was isolated from the mitochondrial fraction of cauliflower inflorescence and characterized.The enzyme is clearly separable from the nuclear DNA polymerases by high salt elution on DEAE-cellulose column chromatography.Template-primer utilization, response to KCl and sensitivity to N-ethylmaleimide of the enzyme are similar to those of DNA polymerase of animal mitochondria.It has been described that mitochondrial DNA polymerases from several different sources of animal tissues or yeast are different in several characteristics from the nuclear fraction (Myer and Simpson

No abstract

No abstract