Currently, therapy with Platelet Rich Plasma (PRP)

Stem cells have a remarkable potential to act as a self renewal in the related system. The proliferation of stem cells can be stimulated by Platelet Rich Plasma (PRP) growth factor. PRP has a potential application for aesthetics, traumatology and maxillofacial surgery. The optimation method could be carried out by a double centrifugation, which is reliable and simple in producing an optimal PRP. The samples were obtained from healthy volunteers; 10 mL of blood was needed to produce 1 mL PRP. CBC test was then performed, in order to know the platelet count before and after double centrifugation. This method used four variations of speed and time. Four variations of speed and time were used to find the optimation result, which yield the highest platelet count and the highest PDGF level released after platelet activation. The measurement of Platelet Derived Growth Factor (PDGF-BB) level was done using ELISA method. Twenty first samples, resulted in a variation of platelet counts, the mean was 0.89 times. About 60% of platelet counts showed a decrease with the lowest value 0.01 times and 40% of the platelet counts showed an increase with the highest value 3.94 times. The study was repeated using “I” tubes, the highest increase of platelet count and PDGF-BB level was obtained by centrifugation at 900 g, 5 minutes duration, then followed by 1500 g, for 15 minutes. In this study protocol, the production of optimal PRP was not yet found, however it provided some important information. In this case, the influence of separation process and skill was more important than the centrifugation speed. The researchers suggested that the measurement of PDGF-BB level should be done immediately after PRP harvest.

Typhoid fever represents an endemic acute infection with a high mortality, in this case a laboratory test is needed to establish theearly diagnosis. For this study the researchers prefer urine diazo test which is a relatively easy, simple and inexpensive test. The aimof this study is to know whether the urine diazo test can be used for screening typhoid fever and whether there is a concordance withthe Widal and TUBEX® TF serological tests. Forty patients aged 2–18 years, attending to the Jeremy Medical Service Clinic, Surabaya,suffering of fever more than 3 days were studied from June up to August 2010. Urine samples were tested by diazo test, while bloodsamples as comparison were tested by Widal and TUBEX® TF tests, and blood culture was carried out as the gold standard. The sensitivityand specificity of those tests were then recorded. From the 40 samples, 12 patients showed positive Salmonella typhi blood cultures,26 diazo positive, 22 Widal positive and 14 TUBEX® TF positive. Of the 12 positive blood cultures, 10 (83%) diazo positive, 7 (58%)Widal positive and 9 (75%) TUBEX® TF positive were found. The sensitivity and specificity of diazo test was 83% and 43%, Widal test58% and 46%, TUBEX® TF test 75% and 82%. It is shown that the Diazo test had a higher sensitivity value, while the TUBEX® TF testshowed a higher level of specificity. In conclusion, so far it can be concluded that the Diazo test is quite reliable in aiding the diagnosisof typhoid fever and can be considered as a screening test for typhoid fever.