The Student Teacher Outreach Mentorship Program (STOMP) at Tufts University brings engineering students to educational settings to support engineering education. In December 2000, Massachusetts incorporated engineering into its state science and technology frameworks. Educators are now working to integrate these new standards into their classroom teachings. Many educators are not familiar with engineering concepts so they are working hard to learn new concepts at the same time as they are implementing projects and teachings to address the frameworks in their settings. STOMP students, engineering undergraduate and graduate students, serve as a support mechanism for these educators by helping students with hands-on projects, resolving technical issues with equipment, answering engineering questions, doing research on topics, and helping to brainstorm activities. The program has been very successful in facilitating engineering education in 10 local classrooms in grades K-9. STOMP studentteacher partnerships have resulted in some fabulous new curriculum units including a 4 th grade unit entitled "Egyptians as Engineers" and a middle school unit entitled "Make your own CD-Learning Digital Logic." The STOMP program also strives to help engineering students understand the educational system and to encourage their involvement, as future members of industry, in K-12 education. This paper will detail the creation and implementation of a project of this nature as well as highlight the difficulties and successes experienced to date.
Arabidopsis END1-LIKE (AtEND1) was identified as a homolog of the barley endosperm-specific gene END1 and provides a model for the study of this class of genes and their products. The END1 is expressed in the endosperm transfer cells (ETC) of grasses. The ETC are responsible for transfer of nutrients from maternal tissues to the developing endosperm. Identification of several ETC-specific genes encoding lipid transfer proteins (LTP), including the END1, provided excellent markers for identification of ETC during seed development. To understand how AtEND1 forms complexes with lipid molecules, a three-dimensional (3D) molecular model was generated and reconciled with AtEND1 function. The spatial and temporal expression patterns of AtEND1 were examined in transgenic Arabidopsis plants transformed with an AtEND1 promoter-GUS fusion construct. The AtEND1 promoter was found to be seed and pollen specific. In contrast to ETC-specific expression of homologous genes in wheat and barley, expression of AtEND1 is less specific. It was observed in ovules and a few gametophytic tissues. A series of AtEND1 promoter deletions fused to coding sequence (CDS) of the uidA were transformed in Arabidopsis and the promoter region responsible for AtEND1 expression was identified. A 163 bp fragment of the promoter was found to be sufficient for both spatial and temporal patterns of expression reflecting that of AtEND1. Our data suggest that AtEND1 could be used as a marker gene for gametophytic tissues and developing endosperm. The role of the gene is unclear but it may be involved in fertilization and/or endosperm cellularization.
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