Hermetia illucens, is used as a reducing agent of palm kernel meal (PKM), as well as one of alternative protein sources for aquaculture purposes. Information about biology of H. illucens is absolutely required in mass production. The objectives of these researches were to study the development of H. illucens including the effect of supplementary food to the adult, and nutrient content of the immature stage. The sample of 20 larvae from each 3 replicates were measured and weighed on 0-19th day (larva) and 24th day (pupa) from egg hatching. H. illucens adults were fed by water and honey 5% (v/v). Eggs were collected and counted. Nutrient content of immature stage: 5, 10, 15, 20 days old (larvae), and 25 days old (prepupae) reared on PKM were analyzed proximately. Dry matter was determined by weight loss on drying at 105 oC during overnight. Crude protein was determined by Kjeldahl procedure (N x 6.25), crude fat by soxhlet (ether extract), crude ash by determining the residue after heating at 550 oC for 4–5 h. Data were analyzed descriptively by average from triplicate. The development of H. illucens was shorter than those in previous studies as the differences of abiotical factor. PKM was a suitable medium for development. It was better, however, to fed the adult with honey since it could enhance the fecundity. The young larva certainly contained the best quality of nutrition. To meet the quantity of mass production, however, the use of the elder larva (bigger) was suggested.

No abstract

Black soldier fly (BSF) larvae (Hermetia illucens) can be used as insect based municipal organic waste recycling technology. The purpose of this research was to examine the utilization of BSF larvae as bio-processors of organic waste in order to produce compost and nutritious larvae biomass. Larvae feeds as treatments of experiment consist of restaurant organic waste mixed with fish silage 6% as treatment A, restaurant organic waste with dairy blood waste 6% as treatment B, and restaurant organic waste as treatment C. The result of this research showed that the highest larvae growth according to its length, width and body weigth was resulted by treatment B, followed by treatments A and C. Total metamorphic development time from egg to imago was 35 days; comprised of as larvae, prepupa, pupa, and imago for 12-13, 6, 6, and 15 days, respectively. Proximate analysis showed that BSF larvae had protein content 33.31 to 33.88%, and fat content 30.07 to 34.39%. Final solid residues of the three treatments was ranged from 90.3-90.6% after 10-11 days of bio-processing.

Ikan sidat tropis (Anguilla spp.) dari Indonesia saat ini menjadi komoditas yang penting di pasar internasional dimikian juga penelitian ikan sidat tropis saat ini menjadi sebuah tantangan baru. Salah satu permasalah utama pada ikan sidat tropis adalah terjadinya tumpang tindih antara karakter morfologi spesies, sehingga identifikasi spesies dengan menggunakan pendekatan morfologi tidak bisa dilakukan. sehingga identifikasi spesies ikan sidat dilakukan dengan pendekatan molekuler. Metode Semi-multiplek PCR yang dikembangkan pada penelitian ini mampu mengidentifikasi tujuh spesies dan subspecies ikan sidatan yang mendiami perairan Indonesia hanya dengan menggunakan satu step PCR. Berdasarkan identifikasi dengan menggunakan semi-multiplek PRC diketahui ada empat spesies ikan sidat yang memiliki penyebaran luas yaitu Anguilla bicolor bicolor, A. b. pacifica, A. marmorata dan A. interioris, dua spesies memiliki penyebaran yang sempit dan mendekati endemis: A. celebesensis dan A. borneensis dan spesies A. nebulosa nebulosa yang hanya ditemukan di perairan barat Indonesia yaitu sungai yang berhubungan dengan Samudera Hindia. Berdasarkan analisis DNA mitokondria gen cytochrome b, tujuh spesies yang mendiami perairan Indonesia memperlihatkan keragaman haplotipe dan nucleotide yang tinggi yaitu masing-masing 0.98 dan 4.57%. Analisa kekerabatan menunjukkan A. borneensis merupakan nenek moyang ikan sidat tropis di perairan Indonesia.

No abstract