Background Health care workers at the frontline are facing a substantial risk of infection during the coronavirus disease 2019 (COVID-19) outbreak. Methods We acquired information and data on the general information, infection and death status of health care workers in Wuhan during the COVID-19 outbreak and completed statistical analyses. Results We have obtained the data on 2,457 infected cases among health care workers in Wuhan, China. More than half of the infected individuals were nurses (52.06%), while 33.62% of infected cases were doctors and 14.33% of cases were medical staff. In particular, the case infection rate of nurses (2.22%) was remarkably higher than that of doctors (1.92%). Most infected cases among health care workers were female (72.28%). A majority of the infected health care workers (89.26%) came from general hospitals, followed by specialized hospitals (5.70%) and community hospitals (5.05%). The case infection rate of health care workers (2.10%) was dramatically higher than that of non-health care workers (0.43%). The case fatality rate of health care workers (0.69%) was significantly lower than that of non-health care workers (5.30%). Conclusions The infection risk of HCWs is clearly higher than that of non-HCWs. HCWs play an essential role in fighting the pandemic. The analysis of the infection status of HCWs is essential to attract enough attention from the public, provide effective suggestions for government agencies and improve protective measures for HCWs.

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The Pi3K/aKT signaling pathway regulates cell proliferation and differentiation in multiple types of cells. The present study aimed to investigate the effects of mechanical stress on c2c12 cell proliferation and to explore the associated mechanisms. a cyclic mechanical stress model of c2c12 myoblasts was established. reverse transcription-quantitative Pcr and western blotting assay were used to examine the Pi3K signaling pathways involved in the progress of cell differentiation. cell counting kit-8 (ccK-8) assay was used to evaluate the proliferation of c2c12 cells. Flow cytometry was employed to evaluate apoptosis following mechanical stress. The results demonstrated that mechanical stress activated the Pi3K signaling pathway in c2c12 myoblasts. Mechanical stress significantly promoted phosphorylation (p-) of AKT and expression of mammalian target of rapamycin (mTor) compared with the normal group. Mechanical stress significantly promoted 4e-binding protein 1 (4eBP1) expression in c2c12 cells compared with the normal group. The PI3K specific inhibitor LY294002 significantly decreased 4eBP1 expression and reduced p-aKT and p-mTor expression compared with the mechanical stress group. Mechanical stress promoted c2c12 cell proliferation. apoptosis of C2C12 significantly decreased in the mechanical stress group compared with the normal group. cyclin d levels significantly increased in the mechanical stress group compared with the normal group. in conclusion, mechanical stress promoted biological functions of c2c12 cells by activating the Pi3K/aKT signaling pathway. These results may contribute to a better understanding of the effects of mechanical stress on cells.

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With a 4-aminostyryl group introduced at its 3-position, a BODIPY BDP-ODTAC was derived as a new ratiometric sensor for Ag by modifying 4-amino group as a Ag chelator, 1-oxa-4,10-dithia-7-azacyclododecane (ODTAC). In addition to the specific Ag-induced hypsochromic absorption shift from 606 to 562 nm, this sensor demonstrated an excitation shift from 600 to 560 nm due to the internal charge transfer (ICT) effect endowed by the introduced α-4-aminostyryl group. The Ag-induced recovery and enhancement of the intrinsic local emission band was also observed. The different sensing behavior of ODTAC-BDP with chelator ODTAC substituting on the meso-phenyl group infers that the ratiometric sensing behavior of BDP-ODTAC is correlated to the amino group in ODTAC acting as the electron donor for the ICT effect. With high Ag selectivity over interfering cations such as Hg and Pb, BDP-ODTAC displays a fluorometric limit of detection (LOD) of ∼17 nM (∼0.002 ppm), which is distinctly lower than EPA and WHO standards for drinking water (500 nM, ∼0.055 ppm). Moreover, the BDP-ODTAC-doped PVC film shows the Ag sensitivity of 1 ppm with a color switch from blue to purple, providing this sensor the ability to determine Ag in totally aqueous solution sensitively via naked-eye detection.