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This contribution describes a simple method for preparing polymeric nanoparticles using photodimerization of anthracene moieties on the side chain of terpolymers in dilute regime and transformation of obtained polymeric nanoparticles into pyrene functional nanoparticles via Menschutkin quaternization procedure. Subsequently, pyrene possessing polymeric nanoparticles are attached onto multiwalled carbon nanotube (MWCNT) surfaces by π-π stacking strategy. Gel permeation chromatography, thermal gravimetric analysis, ultraviolet-visible, and fluorescence spectroscopies are used to analyze modified nanoparticles and their precursors. Electron microscopy and dispersion studies show that pyrene-modified polymeric nanoparticles are able to interconnect various CNTs.Additional supporting information may be found in the online version of this article.

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Phenol is a harmful substance that can be present in many industrial wastewaters. Chemical phenol removal is feasible, but the procedure is challenging and expensive. Biological phenol removal is possible under mild conditions with enzymes. Phenol hydroxylase is the main enzyme for phenol removal. In this study, phenol hydroxylase was induced from filamentous fungus Aspergillus niger. The results have shown that it is possible to use Aspergillus niger as a phenol hydroxylase source, but the enzyme has activity in limited temperature and pH conditions. The optimal working conditions of the enzyme are 30 °C and pH 6.5. The enzyme has moderate storage and thermal stability, and various compounds inhibit it.