Purpose: This study aimed to determine the anti-wrinkle and skin turnover improvement effects of niacinamide-dipeptide convergence (N-peptide). Methods: The following methods were employed to assess the anti-wrinkle and skin turnover improvement effects of N-peptide: 3-(4,5-Dimethylthiazol-2yl)-2,5-diphenyltetrazolium bromide (MTT) assay, wound healing assay, chicken chorioallantoic membrane (CAM) assay, reverse transcription polymerase chain reaction (RT-PCR), and three-dimensional (3D) cell culture. Results: The MTT assay revealed that the N-peptide does not exert a cytotoxic effect in human keratinocytes (HaCaT). Moreover, the scratch wound healing and CAM assays showed that the N-peptide induced migratory properties and angiogenesis, respectively, in HaCaT cells. The expression of matrix metalloproteinases (MMP)-1,-2,-3, and-9 was measured using RT-PCR to evaluate the anti-wrinkle effect of N-peptides. The findings demonstrated that N-peptides were responsible for reducing the expression of the aforementioned MMPs. In addition, using western blotting, N-peptides were identified to increase energy metabolism activity. Furthermore, 3D cell culture analysis indicated improvement in the skin turnover rate because of the N-peptides. Conclusion: Taken together, these results suggest that N-peptides may be a source of cosmetic agents for the improvement of wrinkles and skin turnover.

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