Integrin-based mechanotransduction involves a complex focal adhesion (FA)-associated machinery that is able to detect and respond to forces exerted either through components of the extracellular matrix or the intracellular contractile actomyosin network. Here, we show a hitherto unrecognized regulatory role of vimentin intermediate filaments (IFs) in this process. By studying fibroblasts in which vimentin IFs were decoupled from FAs, either because of vimentin deficiency (V0) or loss of vimentin network anchorage due to deficiency in the cytolinker protein plectin (P0), we demonstrate attenuated activation of the major mechanosensor molecule FAK and its downstream targets Src, ERK1/2, and p38, as well as an up-regulation of the compensatory feedback loop acting on RhoA and myosin light chain. In line with these findings, we show strongly reduced FA turnover rates in P0 fibroblasts combined with impaired directional migration, formation of protrusions, and up-regulation of "stretched" high-affinity integrin complexes. By exploiting tension-independent conditions, we were able to mechanistically link these defects to diminished cytoskeletal tension in both P0 and V0 cells. Our data provide important new insights into molecular mechanisms underlying cytoskeleton-regulated mechanosensing, a feature that is fundamental for controlled cell movement and tumor progression.
Mutations in the cytoskeletal linker protein plectin result in multisystemic diseases affecting skin and muscle with indications of additional vascular system involvement. To study the mechanisms underlying vascular disorders, we established plectin-deficient endothelial cell and mouse models. We show that apart from perturbing the vimentin cytoskeleton of endothelial cells, plectin deficiency leads to severe distortions of adherens junctions (AJs), as well as tight junctions, accompanied by an upregulation of actin stress fibres and increased cellular contractility. Plectin-deficient endothelial cell layers were more leaky and showed reduced mechanical resilience in fluid-shear stress and mechanical stretch experiments. We suggest that the distorted AJs and upregulated actin stress fibres in plectin-deficient cells are rooted in perturbations of the vimentin cytoskeleton, as similar phenotypes could be mimicked in wild-type cells by disruption of vimentin filaments. In vivo studies in endothelium-restricted conditional plectin-knockout mice revealed significant distortions of AJs in stress-prone aortic arch regions and increased pulmonary vascular leakage. Our study opens a new perspective on cytoskeleton-controlled vascular permeability, where a plectin-organized vimentin scaffold keeps actomyosin contractility ‘in-check’ and maintains AJ homeostasis.
The retina of the fat dormouse Glis glis was studied histologically. Opsin immunolabeling identified an unusually dense population of rod photoreceptors (ca. 600,000–780,000/mm²) and a low-density population of L cone photoreceptors containing the longwave-sensitive (LWS) cone opsin, with a shallow maximum of ca. 3,300/mm² in temporal retina and ca. 2,000–3,000/mm² in the remaining retina. Hence the cones comprise only 0.25–0.50% of the photoreceptors. There are no S cones expressing the shortwave-sensitive (SWS1) opsin that is the second cone opsin in most mammals, conveying dichromatic colour vision. We conclude that the fat dormouse is a cone monochromat. Sequencing of the tuning-relevant exon 1 of the SWS1 opsin gene revealed that the gene would have coded for a UV-sensitive visual pigment, but that it contains mutational changes making it nonfunctional. Retinal interneurons (rod bipolar cells, horizontal cells, several amacrine cell types) have rodent-typical features. NeuN-labeled presumed retinal ganglion cells have densities between ca. 4000/mm² in temporal central retina and 850–1300/mm² in peripheral retina. The peak ganglion cell density would result in an estimated visual acuity maximum of ca. 1.8 cycles/degree or ca. 33 minutes of arc. Assessment of a further Gliridae species, the African dormouse Graphiurus sp., also revealed a high rod density, low L cone density, and an absence of SWS1 opsin.
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