ABSTRACT. The objective of this study was to assess the edible biomass and chemical composition of forages grown on pure form, as a grass mix, and in grass-legume consortia. The following species were tested: white oats (Avena sativa), black oats (Avena strigosa), ryegrass (Lolium multiflorum), forage peanut (Arachis pintoi), white clover (Trifolium repens), and red clover (Trifolium pratense). The experiment consisted of sixteen treatments arranged in a completely randomized design. The parameters measured were total dry matter (PMST), neutral detergent fiber (NDF), acid detergent fiber (ADF), and crude protein (CP). No significant differences in PMST were found among the consortia (p > 0.05). Only the pure cultivated white clover (p > 0.05) was comparable to the consortia in terms of biomass production. The three legumes had the lowest average NDF values (p > 0.05), based on their contributions to the total NDF content of the consortia along the cuts. The ADF content increased for all treatments during the cuts. The results indicate that in pasture, legumes increase protein content, and forage consortia increase both the pasture production and the grazing period. Their chemical composition is adequate for boosting livestock production in pastures.Keywords: Arachis pintoi, Avena sp., fiber, Lolium multiflorum, protein, Trifolium sp.Produção e composição bromatológica de gramíneas e leguminosas cultivadas de forma estreme, misturadas ou em consórcio RESUMO. Objetivou-se avaliar a produção de biomassa e a composição bromatológica ao longo do ciclo produtivo de forrageiras cultivadas de forma estreme, misturadas ou consorciadas. Foram utilizadas as seguintes espécies: aveia branca (Avena sativa), aveia preta (Avena strigosa), azevém (Lolium multiflorum), amendoim forrageiro (Arachis pintoi), trevo branco (Trifolium repens) e trevo vermelho (Trifolium pratense). As forrageiras foram cultivadas em 16 tratamentos em delineamento inteiramente casualizado, sendo analisados a produção de matéria seca total (PMST) e os teores de fibra em detergente neutro (FDN), fibra em detergente ácido (FDA) e proteína bruta (PB). Quanto à PMST, não foram constatadas diferenças (p > 0,05) entre os consórcios, sendo apenas o cultivo estreme de trevo branco comparável (p < 0,05) aos consórcios em biomassa produzida. Em relação ao FDN, as três leguminosas isoladas apresentaram os menores valores (p > 0,05) na média geral, ficando evidente a sua contribuição no teor de FDN dos consórcios ao longo dos cortes. O teor de FDA aumentou no decorrer dos cortes para todos os tratamentos. Os resultados indicam que a inclusão de leguminosas aumenta os teores proteicos, e os consórcios de forrageiras aumentam a produção de pastagem e o período de pastejo, com composição bromatológica adequada para potencializar a produção animal a pasto.

Our objective was to quantify the relationship between seasons of the year, milk production, and milk composition of a dairy farm based on data for 48 consecutive months, using multivariate statistical analyses. The dataset contained information on productive indexes and milk composition from the bulk tank milk, which was measured from milk samples, collected monthly and used to determine the total dry extract and defatted dry extract, lactose, fat, protein, somatic cell count, and total bacterial count. Seasons of the year and milk production/hectare were also considered. Factor, cluster, and discriminant analysis were used to study the relationships between the above-mentioned variables. A positive relationship was noted between season and total dry extract, defatted dry extract, milk fat, and protein, with higher values being observed in winter and spring. Similarly, a positive relationship was noted between season and milk production/hectare, lactose content, with an increase in milk production and lactose content in winter and spring, which was negatively related to the somatic cell count and total bacterial count. Milk production and composition varied mainly with seasons. Multivariate analyses facilitated a better understanding of the relationship between milk production and composition on this dairy farm.

This study aimed to evaluate the effects of annatto seeds, linseed oil and their combination on DMI, apparent total tract digestibility, antioxidant capacity and milk composition of dairy cows. Four lactating Holstein cows (120 ± 43 days in milk; 15.98 ± 2.02 kg of milk/day, mean ± SD) were allocated in a 4 × 4 Latin square with a 2 × 2 factorial arrangement (with or without annatto seeds at 15 g/kg of dry matter (DM); with or without linseed oil at 30 g/kg of DM) and provided four different diets: control (no annatto seeds or linseed oil); annatto seeds (15 g/kg of DM); linseed oil (30 g/kg of DM); and a combination of both annatto seeds and linseed oil. Annatto seeds reduced DM intake, and milk yield, protein and lactose, but increased content of fat, total solids and short chain fatty acid, with no effect on total antioxidant capacity of milk. Linseed oil supplementation decreased medium chain fatty acid proportion and n-6/n-3 ratio, conversely it increased long chain fatty acids and n-3 fatty acid content of milk, ether extract intake and total-tract digestibility. Thus, linseed oil supplementation in dairy cow diets improved the milk FA profile but decreased milk fat concentration, whereas annatto seeds did not influence antioxidant capacity and depressed feed intake and milk yield.

Dietary supplementation with royal jelly (RJ) may improve growth, antioxidant gene expression, and intestinal antioxidant capacity, and possibly mitigate effects of stress induced by high stocking density (HSD) in zebrafish (Danio rerio). Effects of HSD and RJ supplementation on growth performance, antioxidant gene expression, and intestinal antioxidant capacity in zebrafish were investigated. A total of 240 four-month-old zebrafish were used in a 2 x 2 factorial design, with four treatments and three replications. The treatments consisted of normal stocking density (NSD) (1 fish/L) and high stocking density (HSD) (3 fish/L) and an unsupplemented commercial diet (0RJ) and commercial diet supplemented with 10% RJ (10RJ). The experiment lasted 25 days. Daily weight gain, final body length, specific growth rate, and survival were recorded. On day 25, six fish per treatment were euthanised and their intestines analysed for antioxidant capacity, and superoxide dismutase 2 (SOD2) and catalase (CAT) gene expression. There were no effects of diet or SD and diet interaction on growth. High stocking density decreased daily weight gain by 25.31%, body length by 2.70%, and survival by 16.85%. Stocking density (SD) and diet interaction influenced intestinal SOD2 and CAT expression and antioxidant capacity. The HSD/0RJ group had lower antioxidant gene expression and antioxidant capacity than the HSD/10RJ and NSD/0RJ groups. Supplementation with RJ did not mitigate the harmful effects of stocking stress. These results indicate that HSD promotes transcriptional suppression of gene encoding enzymes that form the first line of antioxidant defence, resulting in reduced elimination of toxic substances and occurrence of intestinal oxidative stress.

High ambient temperatures and relative humidity affect the behavior and physiology of the animal. This study investigated the influence of different heat-stress-reducing systems on the physiological, behavioral, and preferential responses of Holstein and Jersey cows and heifers on pasture. Experimental treatments were: (1) three heat-stress-reducing systems (sprinklers + artificial shade; showers + artificial shade; and artificial shade); (2) two breeds (Holstein and Jersey); and (3) two physiological stages (lactating cows and pubertal heifers). Physiological and behavioral responses to treatments were measured every 30 min on collection days. The frequency and duration of the use of the systems were recorded continuously 24 h/day for 3 days in each period. The air temperature and the relative humidity were 26 ± 4.1 °C and 74 ± 11.3%, respectively. The experimental treatments affected (p = 0.0354) standing idle, grazing behavior (p = 0.0435), and the frequency and duration of use of the systems by the animals (p < 0.0001). For all treatments, the respiratory rate and the coat surface temperature were highly and significantly correlated (p < 0.05) with the temperature and humidity index. In conclusion, under ambient conditions, dairy cows preferred using sprinklers or showers over artificial shade. These systems were more efficient at reducing the heat load and led to better behavioral and physiological responses.