Various mechanical plus chemical weed control systems for dry bean production were evaluated at Carrington, ND and Crookston and Staples, MN during 1991 and 1992. A dozen tillage plus herbicide weed control systems are available to growers that can provide selective and effective weed control in dry beans without reliance on chloramben—a standard herbicide on dry beans that has been lost. Dry bean yields were similar with low or high level tillage treatments because most of the herbicide treatments with one cultivation gave adequate weed control and any additional tillage did not improve weed control. Kidney bean yields and prices were greater than either pinto or navy beans so the net returns (bean market value minus production costs) were much greater for kidney bean production. Dry bean producers in the North Central part of the U.S. have at their disposal dependable mechanical plus chemical weed control systems, and their market value has been sufficient to make dry bean production very economical in this region.

The prognosis for patients with metastasized prostate cancer is still poor, despite conventional aggressive therapeutic modalities. Several in vitro studies together with animal models and epidemiological studies have indicated that phytochemicals can be antitumorigenic and may be protective against human cancers. However, the potential antitumor effects of genistein isoflavone, a widely studied nutrient phytochemical, have been equivocal. In this study, we investigated the effects of genistein-selenium (Gn-Se) combination on chemosensitivity and matrix metalloproteinase-2 (MMP-2) expression levels in PC3 (hormone-independent) and LNCaP (hormone-dependent) prostate cancer cells. 3-(4,5-Dimethylthiazol-2-yl)-5-(3-carboxymethoxyphenyl)-2-(4-sulfophenyl)-2H-tetrazolium staining and ATP bioassay showed that genistein, selenium, and Gn-Se combination significantly inhibited growth of LNCaP and PC3 cells in a dose- and time-dependent manner, independent of hormonal status, and with no significant differences in chemosensitivity between LNCaP and PC3. Gn-Se combination induced significantly the greatest growth inhibition in both cell lines. Growth inhibition was through apoptosis induction. The treatment-induced apoptotic cascades are caspase-dependent, with evidence of an alternative non-caspase pathway(s). Treatment also induced a dose- and time-dependent decrease in MMP-2 expression levels in PC3 and LNCaP with no significant differences between the two cells. Gn-Se combination induced the greatest depression in MMP-2. Overall, none of the treatment modalities had any significant inhibitory effect in normal prostate epithelial cells. The data obtained from the present study indicate that Gn-Se combination may have chemopreventive value and/or may be adjuvant to standard therapy for prostate tumors independent of hormonal status. MMP-2 expression in cancer cells has been associated with active invasion and metastasis.

Weed removal experiments in dry edible beans were conducted during 1992 and 1993 at Crookston and Staples, MN. Ten manual weed removal treatments were studied to determine when a natural infestation of weeds first reduced dry bean yield, and when weed removal could be discontinued without further loss of seed yield. Major weeds in order of average biomass production on weedy check plots at dry bean harvest over locations and years were wild mustard, foxtail spp., redroot pigweed, common ragweed, wild buckwheat, hairy nightshade, and common lambsquarters. Hairy nightshade also emerged late in the growing season and could negatively affect harvest efficiency and stain navy beans. Weed removal treatments had little effect on dry bean stands or 100-seed weights of harvested dry bean seed. The critical period for weed control in dry beans was 3 to 5 or 6 weeks after planting (WAP). Thus, weed control practices should begin no later than 3 WAP and continue until at least 5 or 6 WAP for maximum dry bean yields.

Weed management experiments with kidney beans were conducted at one irrigated site in 1991 and at two irrigated sites near Staples, MN during 1992. Green foxtail was the most difficult weed to control at the three research sites which agrees with survey results from dry bean growers regarding their worst weed problems. Hairy nightshade increased rapidly when weed management methods were reduced. Dry bean seed yields were highest in cultivated check plots on the farmer's field and in conventional practices plots at the Irrigation Research Center. Net economic returns, averaged over 1991 and 1992, for producing kidney beans at the Irrigation Research Center were $899/ha using conventional practices, $791/ha with cover crop practices, $734/ha with cultivated checks, $690/ha with herbicides only, and $208/ha on weedy checks. Kidney beans are an economical crop for North Central growers, but adequate weed management technologies are necessary for profitable production.

Background: Prostate cancer is the most common form of non-skin cancer within the United States and the second leading cause of cancer deaths. Survival rates for the advanced disease remain relatively low, and conventional treatments may be accompanied by significant side effects. As a result, current research is aimed at alternative or adjuvant treatments that will target components of the signal transduction, cell-cycle and apoptosis pathways, to induce cell death with little or no toxic side effects to the patient. In this study, we investigated the effect of genistein isoflavone, a soy derivative, on expression levels of genes involved in these pathways. The mechanism of genistein-induced cell death was also investigated. The chemosensitivity of the LNCaP prostate cancer cells to genistein was investigated using ATP and MTS assays, and a caspase binding assay was used to determine apoptosis induction. Several molecular targets were determined using cDNA microarray and RT-PCR analysis.Results: The overall data revealed that genistein induces cell death in a time- and dose-dependent manner, and regulates expression levels of several genes involved in carcinogenesis and immunity. Several cell-cycle genes were down-regulated, including the mitotic kinesins, cyclins and cyclin-dependent kinases. Various members of the Bcl-2 family of apoptotic proteins were also affected. The DefB1 and the HLA membrane receptor genes involved in immunogenicity were also up-regulated. Conclusion: The results indicate that genistein inhibits growth of the hormone-dependent prostate cancer cells, LNCaP, via apoptosis induction through regulation of some of the genes involved in carcinogenesis of many tumors, and immunogenicity. This study augments the potential phytotherapeutic and immunotherapeutic significance of genistein isoflavone. Key words: Genistein isoflavone, prostate cancer, expression of genes, phytotherapeutic adjuvant, immunotherapy and chemotherapy