Objective: To assess the religious and spiritual support in critical care according to the conception of patients' family members and nurses. Method: This is a quantitative cross-sectional study conducted with nurses and relatives of patients admitted to intensive care units. Sociodemographic data were collected and a questionnaire on religiosity and spirituality was applied to family members and the Spirituality and Spiritual Care Rating Scale to nurses. The results with p ≤ 0.05 were significant. Results: Sample consisted of intensive care nurses (n = 22) and family members (n = 61). Intensive care nurses stated that they can provide spiritual care (p = 0.03). Of the family members, 88.5% believed that religiosity and spirituality help in coping with difficult times, highlighting prayer as part of care for critically ill patients (p = 0.02). Conclusion: Nurses and family members recognized religious/spiritual support as significant to the process of caring for critically ill patients, even though professional qualification is required for greater integration of this support in daily intensive care.
. Conception, design, intellectual and scientific content of the study; analysis and interpretation of data; manuscript writing; critical revision.
ABSTRACT PURPOSE:To evaluate KGF and human beta defensin-4 (HBD-4) levels produced by dermic fibroblasts and keratinocytes cultivated from burned patients' skin samples.
METHODS:Keratinocytes and fibroblasts of 10 patients (four major burns, four minor burns and two controls) were primarily cultivated according to standard methods. HBD-4 and KGF genes were analyzed by quantitative PCR.
. Conception, design, intellectual and scientific content of the study; analysis and interpretation of data; manuscript writing; critical revision.
Abstract
Purpose:To evaluate the level of cytokines and keratinocyte growth factor (KGF) or Fibroblast Growth Factor 7 (FGF-7) in the culture medium of cultured human dermal fibroblasts from patients with large burn in comparison to small burn.
Methods:Fibroblasts of 10 patients (four large burns, four small burns and two controls) were initiated by the enzymatic method using collagenase. Cytokines and KGF in the supernatant of the culture medium was measured by, respectively, flow cytometry using Cytometric Bead Array Human Inflammation kit (CBA, BD Biosciences, USA) and the enzyme immunoassay method using the Quantikine ® Human KGF. The experiments were performed in triplicate.
Results:The expression of IL-12 protein in patients with large burns showed a tendency to increase. IL-6, IL-10, and IL-1beta were observed no difference. For IL -8, TNF -alpha and KGF was observed a significant difference between the expression in large and small burned patient.
Conclusion:That IL-8, TNF-alpha and KGF showed higher expression in cultured fibroblasts of large burned patients.
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