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The high sensitivity of modern techniques for lifetime detection opens new significant opportunities for investigation of various processes in the biological media. Here, we report on the novel sub-class of cyanine dyes. We examined the effect of electronic coupling on the optical properties of the complex of the dye with the human serum albumin. TCSPC measurements of fluorescence decay provided evidence that the studied dye generates more than two kinds of complexes with albumin. It was established that the effect of “frozen structure” in complex changes the rate of photoisomerization way of the dye’s excited state degradation. Furthermore, by means of molecular docking, the experimentally proposed mechanism of complex formation and the existence of several binding sites were confirmed.

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