Circular RNAs (circRNAs) have been proved to act crucial roles in multiple malignancies including gastric cancer (GC). Retinoic acid induced 14 (RAI14) acts as an oncogene in human cancers, but the underlying mechanisms by which RAI14 is regulated by circRNA/miRNA axis remain elusive. The clinical value of RAI14, miR-23b-3p and circNFATC3 was estimated by The Cancer Genome Atlas and fluorescence in situ hybridization. The interplay between miR-23b-3p and RAI14 or circNFATC3 was determined by qRT-PCR, Western blot, luciferase gene report and RIP assays. Biological function assays and a subcutaneous xenograft model were executed to unveil the role of circNFATC3/miR-23b-3p/RAI14 axis in GC cells. As a consequence, upregulation of RAI14 and circNFATC3 or downregulation of miR-23b-3p was associated with poor prognosis in patients with GC. Restored miR-23b-3p depressed cell proliferation, colony formation, and cell invasion by targeting RAI14, whereas RAI14 facilitated cell progression and reversed the anti-tumor effects of miR-23b-3p in GC cells. Then, circNFATC3 had a co-localization with miR-23b-3p in the cytoplasm in GC tissue cells and could act as a sponge of miR-23b-3p in GC cell line. Silencing of circNFATC3 inhibited cell growth and in vivo tumorigenesis by upregulating miR-23b-3p and downregulating RAI14. In conclusion, our findings indicated that RAI14 facilitated cell growth and invasion and was regulated by circNFATC3/miR-23b-3p axis in GC.

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Purpose: To evaluate the efficacy of Highly Aspherical Lenslets (HAL) in slowing myopia progression among school children and compare it to Orthokeratology. Methods: This retrospective cohort study included 186 cases of myopic individuals aged between 7 to 15 years, with myopia ranging from -0.75 to -8.00D and astigmatism of ≤ 4.0D. Each group consisted of 62 subjects, and only the right eye was included in the analysis. The study involved six-monthly measurements of both cycloplegic refraction and axial length to track changes over time. Results: The baseline average age, gender, Spherical equivalent refraction (SER), and axial length (AL) in the SV group, HAL group, and Ortho-K group were no statistically significant differences (P>0.05). At six months, average (SER) myopia progression was -0.44±0.34 D in the single vision (SV) lenses group and -0.16±0.32 D in the HAL group. Average axial elongation was 0.22±0.13mm in the SV group, 0.08±0.14mm in the HAL group and 0.04±0.11mm in the orthokeratology group. As compared to the SV group, the HAL group reduced myopia progression (SER) to 65.9% (mean difference 0.29±0.46D, P＜0.001). Compared with the SV group, the HAL group axial elongation more slowly(63.6%, mean difference 0.14±0.19mm, P＜0.001). Likewise, axial elongation more slowly at 81.8% for orthokeratology group subjects than the SV group (mean difference 0.18±0.20mm， P＜0.001). In terms of individual issues, 35 of 62 children wearing HAL had no myopia progression after 6 months, higher than the SV group (15 of 62, 24.2%, P＜0 .001). In addition, 30.6% of children with HAL revealed no axial elongation, compared with 3.2% of those with SV lenses (2 of 62, P＜0 .001). The axial elongation of HAL and Ortho-K lenses (28 of 62, 45.2%) was similar (P> 0.05). Conclusions: Wearing the HAL daily slows myopia progression in myopic children and significantly reduces axial elongation. Additionally, both the daily employment of HAL and overnight Orthokeratology demonstrate comparable efficacy in slowing axial elongation in myopic children.

Aim: To develop a new detection technique for ATP in cancer cells using fluorescent biosensing. Materials & methods: This research presents a new label-free fluorescent aptasensor for ATP measurement that incorporates a DNA aptamer, SYBR Gold and single-walled carbon nanohorns. Results: The aptasensor showed selectivity toward ATP and a low limit of detection (37.6 nM). The linear detection range was 100–50,000 nM, and the fluorescence intensity and ATP concentration logarithm showed an excellent linear correlation (R2 = 0.9924). Conclusion: The developed aptasensor may be used to detect cellular ATP in cancer cells and could be employed for biological sample analysis. The benefits of the aptasensor, such as its simplicity, speed, cost–effectiveness, specificity and sensitivity, give it promising implications as a potentially adaptable sensing platform.