No abstract

Human resources is one of the main factor in forest management, so it is important to develop a model of community-based forest management through agroforestry system. The agroforestry system in Trenggalek was implemented by combining a pine plant with Bisi-2 corn varieties. However, the presence of dry pine leaves litter suspected to contain allelopathy, thus make the percentage of germination of seed corn in a low. This study aimed to determine the concentration effect of ethanol extract of pine leaves litter (Pinus merkusii) on the germination of seed corn (Zea mays L.) Bisi-2 varieties. The research design that used Completely Randomized Design and four replications with concentration variations of 0% (control), 10%, 15%, 20% and 25%. Based on ANOVA and Duncan Multiple Range Test with significant level of 5% showed that there was the influence of the ethanol extract of pine leaves litter on the germination of seed corn Bisi-2 varieties. The higher concentration, make the germination of corn seeds more inhibited.

AbstrakPerkembangan penyakit pada manusia saat ini semakin beragam dan berjalan relatif cepat. Salah satu penyebab timbulnya berbagai penyakit tersebut yaitu mikroorganisme seperti bakteri Escherichia coli. Pengobatan yang biasa dilakukan masyarakat yaitu menggunakan obat-obatan kimia, yang dapat menimbulkan efek resisten pada bakteri bahkan efek samping bagi pasien, sehingga penting untuk dilakukan usaha pemanfaatan tanaman sebagai bahan obat herbal dengan memanfaatkan limbah biji buah jeruk manis sebagai antibakteri. Penelitian ini bertujuan untuk mengetahui aktivitas antibakteri biji jeruk (Citrus sinensis) terhadap bakteri Escherichia coli. Jenis penelitian ini adalah eksperimental laboratorium dengan menggunakan metode difusi untuk uji aktivitas antibakterinya. Penelitian dilakukan dengan tahapan sebagai berikut: (1) preparasi sampel berupa serbuk biji jeruk, (2) maserasi serbuk biji jeruk dengan pelarut n-heksana dan etanol, (3) uji antibakteri. Hasil penelitian menunjukkan bahwa biji jeruk menghambat pertumbuhan bakteri E.coli dengan zona hambat ekstrak n-heksan dan etanol sebesar 7,93±1,90 mm dan 18,27±2,40 mm. Biji jeruk manis (Citrus sinensis) memiliki potensi sebagai antibakteri. AbstractDevelopment of disease in humans is increasingly diverse and runs quickly. One cause of the incidence of various diseases are microorganisms such as Escherichia coli. The usual treatment is the use of chemical drugs, which can cause the effects of resistance to bacteria and even side effects for patients, so it is important to do business utilization of plants as herbal medicinal materials by utilizing the waste of sweet orange seeds as an antibacterial. This study aims to determine the antibacterial activity of orange seeds (Citrus sinensis) against Escherichia coli. This study is designed as an laboratory experimental with diffusion method for the antibactey test. The study was conducted with the following stages: (1) preparation of the sample of orange seed powder, (2) maseration of orange seeds powder with n-hexane and ethanol solvent, (3) antibacterial test. The results showed that orange seeds inhibited the growth of Escherichia coli with inhibition zone of n-hexane and ethanol extracts of 7.93±1.90 mm and 18.27±2.40 mm. Sweet orange seeds (Citrus sinensis) have the potential as antibacterial.

Antibacterial Activity of Citrus seed (Citrus reticulata) Extract on Escherichia coli Indonesian agriculture provides a resource of medicinal plants whose potential needs to be explored in order to benefit society. One of them is the use of Siam orange seeds (Citrus reticulata) which has the potential for the production of antibacterial compounds. This study aims to test the antibacterial activity of the ethanol and n-hexane extract of orange seeds. The extract was obtained through maceration techniques using ethanol and n-hexane as solvents. The antibacterial activity test of orange seeds against Escherichia coli used the paper disc diffusion method with nutrient agar (NA) media. The concentration of orange seed extract for the determination of MIC (Minimum Inhibitory Concentration) was 0.5, 2, 8, 10, 20 mg mL-1. The results showed that the ethanol and n-hexane extract of orange seeds had antibacterial activity against E. coli. However, the ethanol extract had a higher antibacterial effect than the n-hexane orange seed extract. From the results of this study, it is hoped that the waste of orange seeds will provide beneficial contribution for pharmaceutical development. Pertanian Indonesia memiliki sumber tanaman obat yang perlu digali potensinya agar bermanfaat bagi masyarakat. Salah satunya pemanfaatan biji jeruk siam (Citrus reticulata) yang berpotensi menghasilkan senyawa antibakteri. Penelitian ini bertujuan untuk menguji aktivitas antibakteri ekstrak etanol dan n-heksana biji jeruk. Ekstrak diperoleh melalui teknik maserasi menggunakan pelarut etanol dan n-heksana. Uji aktivitas antibakteri biji jeruk terhadap Escherichia coli menggunakan metode difusi paper disc dengan media nutrient agar (NA). Konsentrasi ekstrak biji jeruk untuk penentuan MIC (Minimum Inhibitory Concentration) adalah 0,5, 2, 8, 10, 20 mg mL-1. Hasil penelitian menunjukkan bahwa ekstrak etanol dan n-heksana biji jeruk memiliki aktivitas antibakteri terhadap E. coli. Namun, ekstrak etanol memiliki efek antibakteri yang lebih tinggi dibandingkan ekstrak biji jeruk n-heksana. Dari hasil penelitian ini, limbah biji jeruk diharapkan dapat memberikan kontribusi bermanfaat bagi pengembangan farmasi.