This study aimed to optimize an in vitro shootlets regeneration system via direct organogenesis for Phaseolus vulgaris L. cv. Brunca using 24-epibrassinolide (24-Epi). The best medium for shootlets proliferation as well as growth parameters was MS supplemented with 2 mg L-1 TDZ + 0.1 mg L-1 24-Epi. Stem explants recorded the best results of shootlets proliferation percentage compared with leaf or root explants, respectively. MS medium supplemented with 2 mg L-1 TDZ + 0.1 mg L-1 24-Epi gave the best results of recovered shootlets (%); photosynthetic pigments, number of leaves, number of shootlets and shootlet length (cm). From the obtained results it can be recognized that, the behavior and trends of 24-Epi on in vitro P. vulgaris cultures media may be oriented and arranged to the behavior of auxins in the nutrient medium for roots formation and as a cytokinin in shootlets proliferation.