No abstract

Microbiological and Physicochemical studies of abattoir waste was conducted within a period of 12 weeks. Total viable bacterial counts ranged from 4.0x108 to 5.6x108 cfu/ml with cow dung yielding the highest count. Total coliform counts ranged from 1.0x108 to 9.0x108 while total faecal coliform counts ranged from 1.0x108 to 6.0x108 cfu/ml. Twelve Bacteria genera isolated were Escherichia coli, Bacillus cereus, B anthracis, Klebsiella pneumoniae, Staphylococcus aureus, Proteus mirabilis, Salmonella sp, Shigella sonnei, Serratia sp, Pseudomonas aeruginosa, Enterobacter aerogenes, and Lactobacillius sp. With E. coli having 24% occurrence while Enterobacter aerogenes and Pseudomonas had the least occurrence of 2.0 %. The pH of the samples ranged from 6.3 to 8.1 while the Temperature was fairly stable. This study revealed that the abattoir is heavily contaminated with Bacteria and poses public health risk to the populace around the abattoir.

The antimicrobial acts of leaf extracts of Ocimumgratissimum and Prunusamygdalinus were checked on Escherichia coli, Staphylococcus aureus, Pseudomonas aeruginosa and Salmonella typhi using agar cup plate method. The outcomes demonstrated that the chosen organisms were susceptible to the plant extracts at concentrations of 500mg, 50mg and 5mg respectively with diameter zone of inhibition (DZI) of about 12.00± 2.00, 13.00± 2.00, 11.60±3.40, and 12.30±3.30. Phytochemical screening of the leaf extract of Ocimumgratissimum manifested the existence of alkaloid, phenols, glycosides, saponin, steroids and triterpenes while the leaf extract of Prunusamygadalinus demonstrated the existence of saponin, flavonoid and triterpenes. The minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) assayed reveals MIC of 5mg and MBC of 50mg respectively. Ocimumgratissimum and Prunusamygdalinus extracts may be applied to treat disorder caused by these organisms.

Fusariums pecies are opportunistic fungi that play an important role in nosocomial infection. The reservoir of Fusarium species in the hospital is not well understood in our environment. Therefore, the present study sought to identify the reservoir of Fusarium species in hospital environment. Three hundred and sixty (360) samples were collected from the environment of two tertiary health care facilities A and B. The sample consists of water (120), soil (120) and plants (120) which were sourced from hospital environments. Cultures of these samples were performed and polymerase chain reaction was used to confirm Fusarium species. The most predominant specie was Fusarium oxysporum Hospital A:(57.3%) and Hospital B:(64.4%). Most of the Fusarium isolates (76.7%) were recovered from soil samples, followed by water (45.0%) and the least were from plants (30.8%). In conclusion the present study has demonstrated that hospital environment is a reservoir for Fusarium species. However, identification of such reservoir would further enhance effective infection control measures.