The aim of the present study was to evaluate the efficacy of calcium carbonate nanoparticles (CCNPs)/Silver nanoparticles (Ag NPs)/Advanced-PRF composite to induce new bone formation and accelerate bone regeneration when be used to fill an experimentally critical defect in radial bone of rabbits. Twenty-four adult apparently healthy New Zealand white rabbits aged from 5-to 6 months and weighing 3.5 ± 0.5 kg were used in this study and were divided into two groups, group A: was left empty as a control. Group B: we used CCNPs/AgNPs/A-PRF composite. During observation, period rabbits were observed clinically and radiographically at 2,4,8,12 weeks post-operation. Results: the radiographic finding showed a great significant difference between the two groups and the CCNPs/AgNPs/A-PRF composite could make complete healing at the end of the observation period. Conclusion: radiographical studies revealed that CCNPs/AgNPs/A-PRF composite has exalted efficacy to induce new bone formation.