The present study aimed to evaluate the antitumor activity of newly synthesized oxidized form of sodium hypochlorite. Different cell lines were susceptible to Sodium hypochlorite cytotoxicity at acute, LD 50 and therapeutic doses of NaOCl. Therapeutic dose of sodium hypochlorite modulated the significantly reduced level of Hb, IL-12, TNF and the increased levels of creatinine, GPT and GOT towards the normal values. The histopathological study of liver, kidney and spleen groups showed a very significant improvement compared to normal and positive control groups. Sodium Hypochlorite exhibited an anticancer effect on different cell lines in vitro and against EAC in mice.