Piper sylvaticum Roxb., (Family: Piperaceae), commonly known as pahaari peepal, is used in traditional medicine for the treatment of rheumatic pain, headache, asthma, chronic cough, diarrhea, and wounds. To provide scientiic proof for its traditional use, the present study was designed to investigate the antinociceptive and anti-inlammatory properties of methanol extract of P. sylvaticum stem (MEPSS) in pain models. Additionally, computational studies viz. molecular docking, ADME and toxicological property predictions were performed to identify the potent phytochemicals of this plant for antinociceptive and anti-inlammatory activities with good oral bioavailability and safety features. Quantitative phytochemical analysis of MEPSS was performed using established protocols. The antinociceptive activity was determined using acetic acid and formalin test in mice at the doses of 200 and 400 mg/kg while paw edema induced by carrageenan used for anti-inlammatory activity. Molecular docking study was performed by Schrödinger Maestro 10.1 whereas the SwissADME and admetSAR were used for ADME and toxicity prediction respectively. The total phenolic and lavonoid contents of MEPSS were 93.39 and 53.74 mg gallic acid and quercetin equivalent/g of extract respectively. The methanol extract exhibited signiicant and dosedependent antinociceptive and anti-inlammatory efects in experimental pain models. Also, our docking study showed that piperine, piperlonguminine, and sylvamide have the best binding ainities to cyclooxygenase enzymes with good ADME/T properties. This study conirmed that MEPSS possess signiicant antinociceptive and anti-inlammatory activities which could be due to the presence of phytochemicals and three bioactive compounds (piperine, piperlonguminine, and sylvamide) were found to be most efective in computational studies.

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Diabetes mellitus is a metabolic disorder characterized by a relative or total insulin deficit and hyperglycemia (Karunasagara et al., 2020). Diabetic patients suffer from numerous disorders, including vascular illness, like atherosclerosis, neuropathic pain, and diabetes neuropathy (Papatheodorou et al., 2018). According to the report of 2019, 463 million individuals between the ages of 20 and 79 are estimated to have diabetes globally (Saeedi et al., 2019). By 2045, the number of affected people with diabetes is expected to increase to 700 million worldwide. Bangladesh has been in the top ten positions in the world for the number of adults (20-79 years) with diabetes (8.4 million) in 2019. The prevalence of diabetes will be increased to 15.0 million by 2045. Bangladesh has also been ranked nine in 2019 among the countries for 4.7 million adults with undiagnosed diabetes (Atlas, 2015;

The present study sought to evaluate the central nervous system (CNS) depressant, antioxidant, and cytotoxicity activity of methanol and aqueous extract of Trametes versicolor (METV and AETV). The CNS activity was assessed by the open field, hole-cross, forced swimming, thiopental sodium-induced sleeping time, hole-board, and rotarod tests in Swiss albino mice. For both extracts, a substantial decrease in locomotion was observed in open field and hole-cross tests. In addition, the molecular docking study has been implemented through Maestro V11.1. The higher dose of METV (400 mg/kg) and the lower dose of AETV (200 mg/kg) exhibited a significant decrease in immobility time in forced swimming test and increased prolongation of sleep in thiopental sodium-induced sleeping time test, respectively. In contrast, a moderate finding was observed for the hole-board and rotarod tests. Additionally, a significant DPPH scavenging assay and a high toxicity effect in brine shrimp lethality assay were observed. Besides, five phenolic compounds, namely baicalin, quercetin, catechin, p-hydroxybenzoic acid, and quinic acid, were used for the molecular docking study, whereas catechin demonstrated the highest binding affinity towards the targets. The findings conclude that the T. versicolor could be an alternative source for CNS anti-depressant and antioxidant activity.