Background: It is important to adhere to infection control measures in dental procedures due to direct contact with blood and saliva. During the Coronavirus disease 2019 pandemic, regular surveillance is imperative to ensure adherence to the standards. This study aimed to assess the level of adherence to infection control guidelines in specialized dental clinics in different working shifts. Materials and Methods: This study was assessed the level of adherence of 45 selected dental departments to three main guidelines released by (I) the Center for Disease Control (CDC), (II) Ministry of Health (MOH), and (III) armed forces organization (AFO) in three different working shift. The CDC checklist had eight domains. Thus, the items of each domain were individually scored, and considering the weighting coefficient of each item, the total score was calculated. The same procedure was carried out for the two remaining guidelines. Possible effect of working shifts and different specialties on infection control practice was also calculated. Results: The mean rate of adherence was 70.7% to the CDC, 93.8% to the MOH and 84.4% to the AFO guidelines indicating that adherence to the CDC guidelines was lower than the other two domestic guidelines. Individual assessment of each item revealed that hand hygiene (39%) and safe handling and disposal of sharp instruments (46%) acquired the lowest, and sterilization (79%) and safe injection (97%) acquired the highest score according to the CDC checklist. There was no relationship between working shifts and dental specialties regarding the adherence to infection control standards. Conclusion: Dental clinics had different performances regarding infection control guidelines. Further emphasis should be placed on hand hygiene and disposal of sharp instruments.

Introduction: Bacteria almost exclusively cause caries and other oral and dental diseases. This study evaluated the inhibitory effect of the alcoholic extract of Citrullus colocynthis on bacteria and fungi common in oral bacteria diseases. Method and materials: The ethanol extract and essential oil of Citrullus colocynthis were obtained via water distillation. After conducting phytochemical tests, including determination of total phenolic and flavonoid compounds of the extracts, GC-MASS test and microbial tests of the extracts were conducted on S. mutans, E. coli, S. salivarius, L. acidophilus, C. albicans, and S. aureus. The cytotoxicity test was assessed by MTT assay. Results: MIC and MBC values for S. mutans, S. salivalius, and L. acidophilus were 1.56 mg/ml and 3.12 mg/ml, respectively. Moreover, Citrullus colocynthis also has an inhibitory effect on bacteria and C. albicans at these concentrations, according to the DAD test. The survival rate of cells at concentrations of 5 mg/ml and 10 mg/ml was less than 50%, and at concentrations of 2.5 mg/ml and 1.25 mg/ml was less than 70% after 24 hours and 48 hours. Conclusion: According to the cytotoxicity results of this plant at concentrations of 1.56 mg/ml and 3.125 mg/ml, the ethanolic extract of C. colocynthis has inhibitory and lethal effects against pathogenic bacteria and fungi.