The study included clinical investigation on pneumonia that caused by Mannheimia haemolytica (M. haemolytica) in sheep of Basrah Province, also isolation and identification were done and confirmed the diagnosis by PCR technology. The blood samples and nasal swabs were collected from 410 local sheep breeds of both sexes, and different ages. The results showed that from 410 sheep there were 25 healthy against clinical and cultural tests, which concerned as control group. The rest 385 sheep were revealed clinical pneumonia. The most important pneumonic signs included coughing, fever, abnormal lung sounds, dyspnoea, depression, mucopurulent nasal discharge as well as loss of appetite and separated from the herd. The laboratory bacterial culture and biochemical tests for samples from 385 pneumonic sheep appeared M. haemolytica in 81 (21 %) cases, which characterised by moist, round, white or grey colony with β-type haemolysis on blood agar. On MacConkey agar showed pink-red pinpoint colonies. While when stained by gram stain appeared as pink, short rods or coccobacilli and bipolar in methylene blue stain. The biochemical reactions included negative indole, urease and citrate whereas positive for oxidase and catalase tests. The PCR technique indicated that from 81 isolates there were 48 59, 2% cases had evidence by Rpt2 gen as M. haemolytica in local sheep of Basrah Province.
This study was carried out on 250 locally sheep collected from four sides of Thi-Qar Governorate. The clinical, hematological, trace elements changes in naturally occurring minerals deficiency in sheep were described. Anemia & Pale mucous membranes, Alopecia &steely wool, Diarrhea, parakeratosis, pica and abortion constituted the main signs and symptoms. The respiratory and heart rates were significantly higher (p<0.05) in mineral deficient sheep than in normal control sheep in one side of province. Values of total erythrocyte count, hemoglobin concentration and packed cell volume were significantly lower (p<0.05) in mineral deficient sheep than in normal control sheep. Significant differences were not found in total leukocyte count and differential leukocyte count values in sick and normal control sheep. Copper, Zinc and Iron values in the serum were significantly lower (p<0.05) in mineral deficient than in normal control sheep. The erythrocytes morphology appeared a abnormal shapes and size, this refer to Macrocytic hypochromic anemia and Normocytic hypochromic anemia, It was concluded that a significant changes were noticed between diseased and control sheep in clinical hematological values and trace elements and abnormal erythrocytes morphology, deficiency of a single element seldom occurs under field condition in Thi-Qar Governorate.
The present study was conducted to identify the prevalence of brucellosis among buffaloes in Basra governorate, via examination of serum samples from 250 she buffaloes reared in different Basra reigns. Sera were examined firstly by rose Bengal test (RBT) followed by indirect enzyme linked immunsorbent assay (Elisa). The result of RBT indicated that from 250 buffaloes serum samples there were 27(10.8%) positive against Brucella abortus antigen. Elisa test was performed on 88 sera samples that included a 27 RBT positive sera and other 61 negative sera, and the result revealed that 21(23.8%) seropositive sera for Brucella abortus. According to the regions of Basra Governorate the percentage rate of brucellosis were indicated in: Al Hartha 6(5,28%) then Al Qurna 5(4,4%), Al Dear 4(3.5), Al Zubair 3(2.6%), Al Medaina 2(1.76%) and Al Tanooma 1(0.88%). More over, infection in older animals found more significant (P < 0.05) than in youngness, beside that infection rate were high in pregnant buffaloes in compared with non pregnant animals. Conclusion: the brucellosis of buffaloes in Basra governorate were caused by B. abortus and were more prominent in pregnant animals, therefore animals screening of suspected animals was advised,
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