Background: Cervical cancer is a common cancer in women caused by high-risk human papillomavirus (Hr-HPV). Many potential biomarkers have been proposed for precancerous lesions and cancer diagnosis and some of these markers studied for prognosis.This study determined potential biomarkers for cervical cancer diagnosis in regard to HPV genotype by using isobaric labeling quantitative proteomics.Methods: in the current study, there were 75 formalin fixed paraffin embedded (FFPE) uterine cervical samples that used to determine the 14 HPV genotypes and the viral load of each genotype was determined. The tandem mass tag (TMT) proteomic work was performed on four FFPE samples of cervical cancer and four FFPE of control samples. The validation of biomarkers from cervical proteome were evaluated using Immunohistochemistry (IHC) testing. Results:The most frequent HPV genotype among all other genotypes was HPV 16.There were 2753 proteins quantified by TMT and 336 of these proteins had significant differential abundances. KPNA2, MCM2, COL1A1, and DCN were selected based on functional enrichment analysis and validated by Immunohistochemistry (IHC) testing. The staining of IHC confirmed the upregulation of KPNA2 and MCM2 expression in cervical neoplasia and the downregulation of DCN and COL1A1 in some cervical cancer group subjects. Conclusion:The KPNA2 marker was compared to other previously reported biomarkers and is a putative biomarker to be validated in further studies, specifically the relationship with HPV load.

Objectives: The aim of this study was to investigate the prevalence of gastric premalignant changes as gastric atrophy, intestinal metaplasia and dysplastic changes in patients underwent esophagogastroduodenoscopy in three health institutions in Karbala province. Methods: The data was collected from patients attended the endoscopy units during a period from January  to September 2018 in three medical centers in holy Karbala/ Iraq, Al Hussein teaching hospital, Imam Zain Al Abedin hospital and Imam AL Hujjah hospital. It includes 215 males and 285  females who were subjected to esophagogastroduodenoscopy (EGD) from whom mucosal biopsies were taken. These biopsies were stained by Giemsa to detect H. pylori organisms. Results: The mean age of patients was (40.9 +/- 15 years SD). H. Pylori infection was demonstrated in (71%) patients, (19.4%) were presented with glandular atrophy, (7.4%) out of 500 patients had intestinal metaplasia and only 6 (1.2%) patients had dysplastic changes. there was a significant statistical relation between gastric glandular atrophy and age, p value (0.03). there was a significant statistical relation between glandular atrophy and infection with H. Pylori, p value (0.001). there was a significant negative relation between H. Pylori infection and the existence of intestinal metaplasia, p value (0.0001). this study show a significant statistical relationship between the existence of intestinal metaplasia and the occurrence of  dysplastic changes, P value (0.001).  Conclusion: the current study show a significant relation between aging and the existence of gastric glandular atrophy. There was a significant relation between each of gastric glandular atrophy and intestinal metaplastic changes with the H. pylori infection. there was a significant relation between gastric intestinal metaplasia and dysplastic changes.

TOP2A gene status has a significantly positive correlation with HER2/neu status while it has a significantly negative correlation with hormone receptor status.