The current study focuses on the chemical composition, and evaluation of antioxidant and antibacterial activity of the aerial parts of Centaurea resupinata subsp. dufourii. Using different chromatographic methods nine compounds 1-9 were isolated. The structural identification of isolated compounds was achieved using several spectroscopic methods NMR techniques ( 1 H NMR, 13 C NMR, COSY, HSQC, HMBC) and mass spectroscopy (ESI-MS) and by comparison with literature data. The structures of these compounds were identified as nicotiflorin (1), apigetrin (2), chrysoeriol (3), apigenin (4), chrysin (5), daucosterol (6), sitosterol (7), taraxastrerol (8) and lupeol (9).The antibacterial and antioxidant activities of ethyl acetate and n-butanol extracts have been evaluated. The antioxidant activity was assessed in vitro using DPPH radical scavenging method, which showed that ethyl acetate extract possessed an interesting antioxidant potential (IC 50 = 36.263 0.005 μg/mL).

L'enquête ethnobotanique s'avère indispensable pour la connaissance des plantes médicinales et leurs utilisations. Une vaste connaissance de la façon d’utiliser les plantes contre les différentes maladies est fort envisageable dans les zones où l'utilisation des plantes est toujours de grande importance. L’enquête réalisée en 2014 dans les trois wilayas Biskra, El Oued et Ouargla (Sud Est d’Algérie) a révélé que Matricaria pubescens (DESF.) SCHULTZ est utilisée principalement pour le traitement des maladies du tube digestif (53,95%), des affections génito-urinaires (37,96%) et des affections respiratoires (36,34%). L’espèce étudiée est très fréquemment utilisée par les nomades et les villageois. La population enquêtée l’a classé comme plante médicinale. Les résultats de l’enquête ont montré que la partie aérienne constitue la partie la plus utilisée par rapport aux organes souterrains. L’espèce est employée, dans la majorité des cas, sous forme de poudre et en tisane. La poudre est utilisée beaucoup plus par les femmes (34,3 %) que par les hommes (17,44 %). L’utilisation de cette plante est plus élevée chez les personnes âgées (79,37 %). Cette plante est contre-indiquée chez la femme enceinte et en cas d’ulcère gastrique.

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The effect of antioxidant properties as free radical scavenging activity and phytochemical compounds of fresh and dried samples of dill, parsley, coriander, peppermint and celery leaves were studied. Moreover, the chemical constituents and the content of minerals in these medicinal and aromatic plants were also investigated. Results explained that the contents of total phenolics ranged from 1446.88 for coriander to 3128.57mg/100gm for peppermint and after the drying process decreased to 1280.10 and 2356.45mg/100gm, respectively. Also, peppermint had the highest content of flavonoids, while the lowest content was observed in coriander leaves. Otherwise, fresh coriander leaves had the highest content of carotenoids, while the lowest content was in the fresh peppermint leaves. Results explained also that the main component of phenolic compounds was protocatchuic in all fresh and dried samples under investigation. Celery leaves had the highest protocatchuic content. Concerning minerals content reveal that the parsley leaves had the highest content of calcium, potassium and magnesium, while peppermint had the highest phosphorus and iron contents. Results also show that celery leaves had the highest sodium content. Fresh dill and peppermint recorded the highest percentages of total antioxidant activity. Also, fresh celery had gradual effect on DPPH compounds being used as synthetic free radical. Coriander and parsley showed the lowest antioxidant activity. After the drying process total antioxidant activity decreased. Slight decrease was observed with dill and peppermint leaves, while celery and parsley leaves were more effective by using the air oven drier process.