The aim of this study is to determine whether there is a link between the 5.25% sodium hypochlorite irrigation time using Endovac and the endodontic space cleaning quality. The study was done on teeth with a root length of more than 12 mm that did not undergo previous endodontic treatments and had a closed apex. All roots where mechanically prepared and the irrigation protocol was applied acording to the established protocol. The root canal surface was explored by electronic microscopy. A score was given to each image regarding remaining debris and smear layer. The comparative analysis of experimental data shows, that there are no statistically significant differences between the study groups regarding debris and smear layer removal. None of the techniques used in the present study has led to the complete removal of dentinal detritus and root smear layer.

By means of a specific anti-Streptococcus mutans monoclonal antibodies test we want to identify the diabetic patients which have an increased risk to develop the periodontal disease. The highest percentage, of 88.1% of all patients included in this study represents the subjects with a level greater than 500,000 cfu / mL of streptococcus mutans. The Kruskal-Wallis test reveals a value of p = 0.283 resulted from the status of diabetes in patients and the level of streptococcus mutans in saliva. In conclusion, the status of diabetes in patients seems not to influence the salivary level of mutans streptococci determined with the method used in our study.