Background: Understory plants represents the largest component of biodiversity in most forest ecosystems and plays a key role in forest functioning. Despite their importance, the influence of overstory-layer composition on understory plant diversity is relatively poorly understood within deciduous-evergreen broadleaved mixed forests. The aim of this work was to evaluate how tree overstory-layer composition influences on understory-layer diversity in three forest types (monospecific deciduous Nothofagus pumilio (Np), monospecific evergreen Nothofagus betuloides (Nb), and mixed N. pumilio-N. betuloides (M) forests), comparing also between two geographical locations (coast and mountain) to estimate differences at landscape level. Results: We recorded 46 plant species: 4 ferns, 12 monocots, and 30 dicots. Canopy-layer composition influences the herb-layer structure and diversity in two different ways: while mixed forests have greater similarity to evergreen forests in the understory structural features, deciduous and mixed were similar in terms of the specific composition of plant assemblage. Deciduous pure stands were the most diverse, meanwhile evergreen stands were least diverse. Lack of exclusive species of mixed forest could represent a transition where evergreen and deciduous communities meet and integrate. Moreover, landscape has a major influence on the structure, diversity and richness of understory vegetation of pure and mixed forests likely associated to the magnitude and frequency of natural disturbances, where mountain forest not only had highest herb-layer diversity but also more exclusive species. Conclusions: Our study suggests that mixed Nothofagus forest supports coexistence of both pure deciduous and pure evergreen understory plant species and different assemblages in coastal and mountain sites. Maintaining the mixture of canopy patch types within mixed stands will be important for conserving the natural patterns of understory plant composition in southern beech mixed forests.

Significant gaps remain in understanding the response of plant reproduction to environmental change. This is partly because measuring reproduction in long-lived plants requires direct observation over many years and such datasets have rarely been made publicly available. Here we introduce MASTREE+, a data set that collates reproductive time-series data from across the globe and makes these data freely available to the community. MASTREE+ includes 73,828 georeferenced observations of annual reproduction (e.g. seed and fruit counts) in perennial plant populations worldwide.These observations consist of 5971 population-level time-series from 974 species in 66 countries. The mean and median time-series length is 12.4 and 10 years respectively, and the data set includes 1122 series that extend over at least two decades (≥20 years of observations). For a subset of well-studied species, MASTREE+ includes extensive replication of time-series across geographical and climatic gradients. Herewe describe the open-access data set, available as a.csv file, and we introduce an associated web-based app for data exploration. MASTREE+ will provide the basis for improved understanding of the response of long-lived plant reproduction to environmental change. Additionally, MASTREE+ will enable investigation of the ecology and evolution of reproductive strategies in perennial plants, and the role of plant reproduction as a driver of ecosystem dynamics.

& Key message Canopy composition in mixed Patagonian Nothofagus forests has an impact on soil properties like cationic exchange capacity and pH, while most soil variables changed with site [P, cation exchange capacity (CEC), soil moisture (SM), resistance to penetration (R)]. Site conditions and canopy composition need be considered together to better understand the distribution of Nothofagus species. & Context Forests can influence soil development, changing main soil properties by several mechanisms, but this has never been investigated in mixed Nothofagus forests. Such influences can be strongly variable depending on local site conditions, and the interactions between both factors need to be clearly established. & Aims To reveal the effect on physical and chemical mineral soil properties in mixed Nothofagus forests of canopy composition and their interactions with site conditions. & Methods Two sites and three mixed forest types, with different proportion of deciduous-evergreen, were selected in coastal and mountain sites. We evaluated forest structure, litterfall and physico-chemical properties of mineral soil (N = 60), and analyzed these factors with general linear models and multivariate statistics [multi-response permutation procedure (MRPP), principal component analyses (PCA)]. & Results Basal area and litterfall were significantly influenced by forest type × sites. CEC and pH value varied among forests types and sites, but sites also influenced other variables (P, SM, R). Multivariate analyses reveals a much stronger effect of site conditions than that of species mixture. & Conclusion Forest soil studies must consider environmental factors on different spatial scale and canopy species composition to better understand their influence on soil dynamics in mixed Nothofagus forests.