Ciprofloxacin comprise a medically effective and generally utilized class of broad-spectrum antibiotics; as a result of the intensive use of this type of therapy, bacteria have become resistant, lead to minimize the usage of such bactericides in field of management as well as treatment of microbial illness. Pseudomonas aeruginosa one of important pathogens that case wide spectrum of infections, the evolution of ciprofloxacin-resistant Pseudomonas aeruginosa were notify. In this investigation 50 clinical isolates of Pseudomonas aeruginosa were collected, antibiotic susceptibility test for ciprofloxacin by using the Kirby-Bauer technique as well as MIC was performed to all these isolates. Specific primers sequences for detection of Topoisomerase II (gyrA) genes were used by conventional PCR then, PCR product were treated with Sac II enzyme at 37°C for 2 h, then restriction bands were recovered by gel electrophoresis. From Fifty Pseudomonas aeruginosa isolates enrolled in this study, 33(66%) show sensitive to Ciprofloxacin, 17(34%) reported resistant to Ciprofloxacin. RFLP-PCR showed that out of 17 isolates resistant to Ciprofloxacin mutation inDNA gyrase (GyrA) were detected in six (35.2 %) isolated while the reminder 11 (64.7%) has no mutation. The presence of mutations in Topoisomerases II genes mediate Ciprofloxacin defeat as therapy for Pseudomonas aeruginosa. The current venture, were made to demonstrate Topoisomerase II genes (GyrA mutations in Pseudomonas aeruginosa that could be responsible for molecular mechanism of Ciprofloxacin resistance.

Introduction: Toxoplasma infection was higher in infertile couples than fertile couples, probably due to anti-sperm antibodies that were higher in couples with Toxoplasmosis. Investigations of T. gondii infections in men with infertility showed that among 100 cases of men's infertility, 36% were serologically positive for Toxoplasma-IgG and IgM. It has been concluded that T. gondii can affect men's fertility and result in infertility. Materials and Methods: Selective infertile males were asked about days of sexual abstinence. Seminal fluid samples were collected following a minimum of 2 days and a maximum of 7 days from abstinence. Every patient was given a clean, wide mouth, sterile, dry, graduated plastic and warm disposable container. The samples were obtained by masturbation in a private room near the semen analysis lab to reduce seminal exposure to temperature fluctuations and control the time from collection to analysis. Results: For the ND1 gene, samples of 8 different fertility groups have been sequenced. These sequences have been compared to reference sequences taken from the NCBI database. Several mutations in various nucleotide positions of the ND1 regions have been detected in samples from multiple groups. The base substitution has been positioned on the nucleotides (nts) 3480, 3567, 3591, 3693, and 4216. The T to C evolution was notorious at nt 3480 in ND1 genes. The SNP was detected in an asthenospermia human (Sample code: 010480). Keywords: Sequence, ND1 gen, Oligospermia Toxoplasmosis, Couples infertility

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