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Narcissus (Narcissus albidus) imported from the United States exhibited leaf chlorosis during post-entry quarantine. We employed next-generation sequencing (NGS) on symptomatic leaf samples and detected vallota mosaic virus (ValMV) belonging to the genus Potyvirus, family Potyviridae, as the viral agent. Sanger sequencing of PCR and rapid ampli cation of cDNA ends based on NGS contigs revealed that ValMV was 9,451 nucleotides (nt) in length, excluding the poly(A) tail. Nucleotide and amino acid (aa) sequences of the coat protein region had over 98% identity to previously reported ValMV isolates. At each of the 10 mature protein regions, however, sequence identity with other potyviruses was 49.5-71.9% nt and 18.3-78.9% aa, values that are below the species demarcation criteria for Potyviridae. Phylogenetic

The anemone mosaic virus (AnMV) and ranunculus mild mosaic virus (RanMMV) infects the anemone plant with characteristic mosaic patterns on leaves. Two complete genome sequences of the two viruses imported from the Netherlands, were determined based on deep sequencing for the first time. Each of AnMV and RanMMV had 9,698 and 9,537 nucleotides (nt), excluding the poly(A) tail. They shared 80.0% nt/amino acid (aa) sequence identities or more, which are above the species demarcation value, with only AnMV and RanMMV reported previously in coat protein region, but having 68.0% nt/aa sequence identities or less with other potyviruses in each coding region of the complete sequences. Additionally, phylogenetic analysis showed that AnMV and RanMMV were included in other known potyviruses. These results suggest that both of AnMV and RanMMV were independent species belonging to the genus Potyvirus.

Narcissus (Narcissus albidus) imported from the United States exhibited leaf chlorosis during post-entry quarantine. We employed next-generation sequencing (NGS) on symptomatic leaf samples and detected vallota mosaic virus (ValMV) belonging to the genus Potyvirus, family Potyviridae, as the viral agent. Sanger sequencing of PCR and rapid amplification of cDNA ends based on NGS contigs revealed that ValMV was 9,451 nucleotides (nt) in length, excluding the poly(A) tail. Nucleotide and amino acid (aa) sequences of the coat protein region had over 98% identity to previously reported ValMV isolates. At each of the 10 mature protein regions, however, sequence identity with other potyviruses was 49.5–71.9% nt and 18.3–78.9% aa, values that are below the species demarcation criteria for Potyviridae. Phylogenetic analysis revealed that our ValMV isolate is most closely related to known ValMV and is grouped within other potyviruses. Taken together, our results indicate that the newly isolated ValMV belongs to a distinct species of Potyvirus. This study provides the first report of the complete ValMV genome sequence and the first record of this virus from the narcissus.