Background: In cervical cancer, more than any other type of cancer, the effects of prevention, early diagnosis and prompt treatment is apparent on reduction of mortality. Objectives: This study aims to achieve protein biomarkers which can be useful in diagnosis and treatment of cervical cancer. Methods: In this Descriptive study, out of 3 patients who had a positive HPV PCR test, with symptoms of cervical tumor, samples of healthy and cancerous tissues were obtained to perform proteomics. By standard separation techniques, total tissue proteins were purified and separated by using two-dimensional electrophoresis. Results: Proteomes of healthy and cancerous tissues were compared with each other and the level of expression of considered proteins was evaluated using the required analyses. A total of 173 proteins in gels of two-dimensional electrophoresis was determined, 37 of which had a significant difference in expression. Because descriptive essay. We compared and statistical tests do not make sense. Conclusions: It seems that in this disease, the expression of a large number of tissue proteins in cancerous tissue is changed, and most of them reduced or not expressed at all. These changed proteins can be useful as a marker in identification and treatment of this disease.