No abstract

Three Gram-negative, motile, mesophilic, aerobic, rod-shaped bacterial strains, designated 2O1T, 1O14 and 1O18, were isolated from Indonesian seawater after enrichment with crude oil and a continuous supply of supplemented seawater. The strains exhibited high n-alkane-degrading activity, which indicated that the strains were important degraders of petroleum aliphatic hydrocarbons in tropical marine environments. Phylogenetic analyses based on 16S rRNA gene sequences of members of the Gammaproteobacteria showed that the isolates formed a coherent and distinct cluster in a stable lineage containing Oceanobacter kriegii IFO 15467T (96.4–96.5 % 16S rRNA gene sequence similarity) and Thalassolituus oleivorans MIL-1T. DNA G +C content was 53.0–53.1 mol%. The major fatty acids were C16 : 0, C16 : 1 ω7 and C18 : 1 ω9 and the hydroxy fatty acids were C12 : 0 3-OH and C10 : 0 3-OH. The polar lipids were phosphatidylglycerol, a ninhydrin-positive phospholipid(s) and glycolipids. The major quinone was Q-9 (97–99 %), which distinguished the isolates from Oceanobacter kriegii NBRC 15467T (Q-8; 91 %). On the basis of phenotypic, genotypic and chemotaxonomic data, including DNA–DNA hybridization, the isolates represent a novel genus and species, for which the name Oleibacter marinus gen. nov., sp. nov. is proposed. The type strain of Oleibacter marinus is 2O1T (=NBRC 105760T =BTCC B-675T).

Tissue conditioners are temporary materials used to improve the health of the soft tissues of the denture-bearing areas. They are soft and flexible, and because of their pliability, they allow the bruised and distorted soft tissues to return to their normal size and shape, as well as reduce inflammation and swelling. In 1991, the International Standard ISO 10139-1 was established by Technical Committee ISO/TC 106, Dentistry, SubCommittee SC 2, Prosthodontic Materials. The purpose of this study was to evaluate the physical and mechanical properties of some tissue conditioners using this standard test, and also to analyze the composition of these materials: The results were as follows: 1. Consistency test All materials in this study agreed with ISO standards. 2. Penetration test Some materials, after 2 hours, were below these requirements, and it seemed that the results were influenced by concentration of ethanol. After 1 week, all tested materials fell within this standard. 3. There was evidence of a correlation between the consistency and the concentration of ethanol.