No abstract

Iamia majanohamensis gen. nov., sp. nov., an actinobacterium isolated from sea cucumber Holothuria edulis, and proposal of Iamiaceae fam. nov. Kurahashi & Yokota, 2007b). In this study we used SN medium, which is commonly used for algal cultivation, and a novel bacterial strain (F12 T ), forming a deep branch with Acidimicrobium ferrooxidans in the order Acidimicrobiales, was isolated.Acidimicrobium strains are Gram-positive, rod-shaped bacteria that are found in warm, acidic, sulphur-or mineral sulphide-rich environments. Acidimicrobium ferrooxidans was originally described by Clark & Norris (1996) and was subsequently placed in the subclass Acidimicrobidae (Stackebrandt et al., 1997), which contains only the order Acidimicrobiales, family Acidimicrobiaceae, genus Acidimicrobium and one species, Acidimicrobium ferrooxidans. Interestingly, Rheims et al. (1999) carried out molecular environmental studies and discovered the existence of several novel 16S rRNA gene sequence clusters with probable worldwide distribution in different terrestrial environments, forming several lineages deeply rooted to the order Acidimicrobiales. However, no novel species belonging to these clusters have been reported.The strain used in this study was obtained from the abdominal epidermis of a sea cucumber, Holothuria edulis, which was collected off the coast of Aka Island, Okinawa prefecture, Japan, at a depth of 6 m, as described previously (Kurahashi & Yokota, 2004). The medium used for isolation of strain F12T was SN medium, consisting of Abbreviations: DAP, diaminopimelic acid; ML, maximum likelihood; NJ, neighbour joining; TEM, transmission electron microscopy.The GenBank/EMBL/DDBJ accession number for the 16S rRNA gene sequence of strain F12 T is AB360448.A supplementary figure showing the general morphology of cells of strain F12 T is available with the online version of this paper.

Three Gram-negative, motile, mesophilic, aerobic, rod-shaped bacterial strains, designated 2O1T, 1O14 and 1O18, were isolated from Indonesian seawater after enrichment with crude oil and a continuous supply of supplemented seawater. The strains exhibited high n-alkane-degrading activity, which indicated that the strains were important degraders of petroleum aliphatic hydrocarbons in tropical marine environments. Phylogenetic analyses based on 16S rRNA gene sequences of members of the Gammaproteobacteria showed that the isolates formed a coherent and distinct cluster in a stable lineage containing Oceanobacter kriegii IFO 15467T (96.4–96.5 % 16S rRNA gene sequence similarity) and Thalassolituus oleivorans MIL-1T. DNA G +C content was 53.0–53.1 mol%. The major fatty acids were C16 : 0, C16 : 1 ω7 and C18 : 1 ω9 and the hydroxy fatty acids were C12 : 0 3-OH and C10 : 0 3-OH. The polar lipids were phosphatidylglycerol, a ninhydrin-positive phospholipid(s) and glycolipids. The major quinone was Q-9 (97–99 %), which distinguished the isolates from Oceanobacter kriegii NBRC 15467T (Q-8; 91 %). On the basis of phenotypic, genotypic and chemotaxonomic data, including DNA–DNA hybridization, the isolates represent a novel genus and species, for which the name Oleibacter marinus gen. nov., sp. nov. is proposed. The type strain of Oleibacter marinus is 2O1T (=NBRC 105760T =BTCC B-675T).