Iamia majanohamensis gen. nov., sp. nov., an actinobacterium isolated from sea cucumber Holothuria edulis, and proposal of Iamiaceae fam. nov.

Kurahashi, Midori; Fukunaga, Yukiyo; Sakiyama, Yayoi; Harayama, Shigeaki; Yokota, Akira

doi:10.1099/ijs.0.005611-0

Cited by 81 publications

(61 citation statements)

References 23 publications

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“…This confirms and extends our previous finding of novel Acidimicrobidae in Xestospongia sponges (27). The clones are distantly related to several cultured isolates from activated sludge, soil, various marine environments, and one newly described species, Iamia majanohamensis, isolated from a sea cucumber (15).…”

Section: Discussionsupporting

confidence: 77%

Sponge-Associated Bacteria Are Strictly Maintained in Two Closely Related but Geographically Distant Sponge Hosts

Montalvo

Hill

2011

Appl Environ Microbiol

110

103

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The giant barrel sponges Xestospongia muta and Xestospongia testudinaria are ubiquitous in tropical reefs of the Atlantic and Pacific Oceans, respectively. They are key species in their respective environments and are hosts to diverse assemblages of bacteria. These two closely related sponges from different oceans provide a unique opportunity to examine the evolution of sponge-associated bacterial communities. Mitochondrial cytochrome oxidase subunit I gene sequences from X. muta and X. testudinaria showed little divergence between the two species. A detailed analysis of the bacterial communities associated with these sponges, comprising over 900 full-length 16S rRNA gene sequences, revealed remarkable similarity in the bacterial communities of the two species. Both sponge-associated communities include sequences found only in the two Xestospongia species, as well as sequences found also in other sponge species and are dominated by three bacterial groups, Chloroflexi, Acidobacteria, and Actinobacteria. While these groups consistently dominate the bacterial communities revealed by 16S rRNA gene-based analysis of sponge-associated bacteria, the depth of sequencing undertaken in this study revealed clades of bacteria specifically associated with each of the two Xestospongia species, and also with the genus Xestospongia, that have not been found associated with other sponge species or other ecosystems. This study, comparing the bacterial communities associated with closely related but geographically distant sponge hosts, gives new insight into the intimate relationships between marine sponges and some of their bacterial symbionts.Marine sponges (phylum Porifera) are ancient metazoans with a fossil record dating back over 600 million years, but the duration of the sponge-bacterium association is less well documented. Sponges actively pump seawater through pores and channels to obtain food consisting mainly of microbes filtered from the surrounding seawater, but they have no true organs or circulatory, nervous, or digestive systems. Bacterial symbionts may play roles in digestion, waste removal, and chemical defense for the sponge host (38). We use the terms "symbiont" and "symbiosis" in the same sense as Taylor et al. (38) and consistent with the original definition of de Bary to refer to two or more organisms consistently found living together for a long period without any implication that the organisms benefit or harm each other.Many sponges contain chemical compounds with bioactive properties, several of which are promising as pharmaceutical leads, and sponge-associated microorganisms are of interest due to their potential role in producing some of these compounds (10). Studies of the bacteria associated with marine sponges have revealed novel bacterial groups, such as the candidate phylum "Poribacteria" (6, 16), as well as ubiquitous symbionts found in many different sponges, including the NW001-like Alphaproteobacteria (4, 46). Sponge-microbe associations provide a model for studying ancient, complex symbioses ...

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Section: Discussionsupporting

confidence: 77%

Sponge-Associated Bacteria Are Strictly Maintained in Two Closely Related but Geographically Distant Sponge Hosts

Montalvo

Hill

2011

Appl Environ Microbiol

110

103

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“…Strain F10 T was isolated from the abdominal epidermis of a sea cucumber, Holothuria edulis, which had been collected off the coast of Aka Island, Okinawa prefecture, Japan, at a depth of 6 m. The novel marine actinobacterium, Iamia majanohamensis F12 T , was isolated from the same specimen (Kurahashi et al, 2009). Strain F10 T was isolated on SN medium agar plates as described by Kurahashi et al (2009) after incubation at 25 u C for approximately 5 weeks.…”

mentioning

confidence: 99%

“…Strain F10 T was isolated on SN medium agar plates as described by Kurahashi et al (2009) after incubation at 25 u C for approximately 5 weeks. Subcultivation of the isolate was carried out on marine agar 2216 (MA; Becton Dickinson) at 20 u C. Stock cultures of the isolate in marine broth 2216 (Becton Dickinson) with 5 % DMSO were preserved at 280 u C.…”

mentioning

confidence: 99%

Euzebya tangerina gen. nov., sp. nov., a deeply branching marine actinobacterium isolated from the sea cucumber Holothuria edulis, and proposal of Euzebyaceae fam. nov., Euzebyales ord. nov. and Nitriliruptoridae subclassis nov.

Kurahashi

Fukunaga

Sakiyama

et al. 2010

International Journal of Systematic and Evolutionary Microbiology

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Euzebya tangerina gen. nov., sp. nov., a deeply branching marine actinobacterium isolated from the sea cucumber Holothuria edulis, and proposal of Euzebyaceae fam. nov., Euzebyales ord. nov. and Nitriliruptoridae subclassis nov. A tangerine-coloured, Gram-positive actinobacterial strain, designated F10 T , was isolated from the abdominal epidermis of a sea cucumber, Holothuria edulis, collected in seawater off the coast of Japan. A 16S rRNA gene sequence analysis indicated that strain F10 T was a member of the class Actinobacteria and was most closely related to Nitriliruptor alkaliphilus ANL-iso2 T (87.4 % sequence similarity). Phylogenetic analyses showed that strain F10 T represented a novel, deeprooted, and distinct phylogenetic lineage within the class Actinobacteria and clustered with N. alkaliphilus and uncultured bacteria. The organism had meso-diaminopimelic acid as the diagnostic diamino acid in the cell-wall peptidoglycan, and rhamnose and galactose as the diagnostic cell-wall sugars. Strain F10 T contained C 16 : 1 v7c, C 16 : 0 and C 17 : 1 v8c as the major cellular fatty acids. The predominant isoprenoid quinone was MK-9 (H 4 ). The G+C content of the DNA was 68.3 mol%. Based on data from the current polyphasic study, it is proposed that the new marine isolate be placed in a novel genus and be considered a novel species designated Euzebya tangerina gen. nov., sp. nov. within the new family, order and subclass Euzebyaceae fam. nov., Euzebyales ord. nov. and Nitriliruptoridae subclassis nov. in the class Actinobacteria. The type strain of Euzebya tangerina is F10 T (5NBRC 105439 T 5KCTC 19736 T ).In the course of searching for novel lineages of bacteria from the intestine and epidermis of marine creatures, a novel bacterial strain, F10 T , was isolated, and this strain was shown to form a deep branch in the class Actinobacteria. As the field of marine actinobacterial research is still in its early stages (Bull et al., 2005), the isolation and taxonomic characterization of strain F10 T promises to increase our understanding of marine actinobacteria. Stackebrandt et al. (1997) proposed a hierarchical classification system for the actinobacteria by conducting 16S rRNA gene sequence analyses and by distinguishing between patterns of signature nucleotides. As a result, the phylum Actinobacteria was classified into 5 subclasses within one class Actinobacteria Stackebrandt et al. 1997: Acidimicrobidae Stackebrandt et al. 1997, Rubrobacteridae Stackebrandt et al. 1997, Coriobacteridae Stackebrandt et al. 1997, Actinobacteridae Stackebrandt et al. 1997 and Sphaerobacteridae Stackebrandt et al. 1997. Sphaerobacter thermophilus was originally described by Demharter et al. (1989) and was subsequently placed in the subclass Sphaerobacteridae, which contains only the order Sphaerobacterales, the family Sphaerobacteraceae, the genus Sphaerobacter, and one species, S. thermophilus. More recently, Hugenholtz & Stackebrandt (2004) proposed the reclassification of S. thermophilus from the subclass Sphaerobacteridae...

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“…In an attempt to isolate various thermoacidophilic microorganisms from a solfataric field in Hakone, Japan (Itoh et al, 2007), a novel thermoacidophilic bacterial strain, designated IC-180 T , which was phylogenetically affiliated with acidophilic actinobacteria in the order Acidimicrobiales (Stackebrandt et al, 1997;Zhi et al, 2009), was isolated. At the time of writing, the order Acidimicrobiales comprises two families, Acidimicrobiaceae and Iamiaceae (Zhi et al, 2009;Kurahashi et al, 2009). The former family comprises three monospecific genera, Acidimicrobium (Clark & Norris, 1996), Ferrimicrobium and Ferrithrix (Johnson et al, 2009), members of which are found in mineral-rich acidic environments such as mines and solfataric fields.…”

mentioning

confidence: 99%

Aciditerrimonas ferrireducens gen. nov., sp. nov., an iron-reducing thermoacidophilic actinobacterium isolated from a solfataric field

Itoh

Yamanoi

Kudo

et al. 2011

International Journal of Systematic and Evolutionary Microbiology

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Iamia majanohamensis gen. nov., sp. nov., an actinobacterium isolated from sea cucumber Holothuria edulis, and proposal of Iamiaceae fam. nov.

Cited by 81 publications

References 23 publications

Sponge-Associated Bacteria Are Strictly Maintained in Two Closely Related but Geographically Distant Sponge Hosts

Sponge-Associated Bacteria Are Strictly Maintained in Two Closely Related but Geographically Distant Sponge Hosts

Euzebya tangerina gen. nov., sp. nov., a deeply branching marine actinobacterium isolated from the sea cucumber Holothuria edulis, and proposal of Euzebyaceae fam. nov., Euzebyales ord. nov. and Nitriliruptoridae subclassis nov.

Aciditerrimonas ferrireducens gen. nov., sp. nov., an iron-reducing thermoacidophilic actinobacterium isolated from a solfataric field

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