Madhuca longifolia, Cassia tora, Cocculus hirsutus, Celosea argentea and Alternanthera sessilis plants were scrutinized to determine the amino acid content by a validated method. Herein we report a novel, accurate and cost‐effective reversed‐phase high‐performance liquid chromatography method for the simultaneous determination and quantification of 14 amino acids. The developed method was validated as per the International Conference on Harmonisation guidelines. All calibration curves showed a good linear relationship (r > 0.9928) within test range. Precision was assessed by intra‐ and inter‐day tests with relative standard deviations less than 2%. Recovery of 98.38–99.60% with relative standard deviations <1.00% was observed. On quantification, glutamic acid, aspartic acid, alanine, methionine, glycine, leucine, valine and threonine were present in major quantity from different parts of selected plants which is advantageous as they control most of the physiological activities of the body. The proposed method can be used for the simultaneous estimation of 14 amino acids in one run and can be extended for amino acid‐based standardization of herbal formulations containing these plants and its use in pharmaceutical industries.

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Background: The present study was carried out for determination of amino acid content in tubers of Dioscorea bulbifera using reverse-phase high-performance liquid chromatography. Methods: The method involved the vapor phase hydrolysis of the sample, automated derivatisation of the amino acids with the aid of AccQ-Fluor reagent kit, separated on a high performance liquid chromatography equipped with photo diode array (HPLC-PDA) at 254 nm having column temperature of 37 ºC. Results: The proportional molar concentration for each amino acid was calculated based on the concentration of standard amino acids and expressed as μg amino acid/mg sample. Methionine, aspartic acid and leucine were major components while as tyrosine was found minor from the plant on dry weight basis. Conclusion: The method is reliable, simple and economical for determining the amino acid content of Dioscorea bulbifera tubers.

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