Two dimensional (2D) cell culture systems lack the ability to mimic in vivo conditions resulting in limitations for preclinical cell-based drug and toxicity screening assays and modelling tumor biology. Alternatively, 3D cell culture systems mimic the specificity of native tissue with better physiological integrity. In this regard, microfluidic chips have gained wide applicability for in vitro 3D cancer cell studies. The aim of this research was to develop a 3D biomimetic model comprising culture of breast cancer cells in butterfly-shaped microchip to determine the cytotoxicity of carnosic acid and doxorubicin on both estrogen dependent (MCF-7) and independent (MDA-MB231) breast cancer cells along with healthy mammary epithelial cells (MCF-10A) in 2D, 3D Matrigel™ and butterfly-shaped microchip environment. According to the developed mimetic model, carnosic acid exhibited a higher cytotoxicity towards MDA-MB 231, while doxorubicin was more effective against MCF-7. Although the cell viabilities were higher in comparison to 2D and 3D cell culture systems, the responses of the investigated molecules were different in the microchips based on the molecular weight and structural complexity indicating the importance of biomimicry in a physiologically relevant matrix.

Material science and engineering are the sources of divergent emerging technologies, since all the modifications and developments are being made to reach a novel biomaterial to fulfill the requirements of biomedical applications, the first important feature is the biocompatibility of the new advanced material. In this chapter, the general biocompatibility concept, test systems to determine biocompatibility, examples of bioinspired materials and their altered biocompatibility and future expectations from these novel bioinspired materials will be discussed.

Material science and engineering are the sources of divergent emerging technologies, since all the modifications and developments are being made to reach a novel biomaterial to fulfill the requirements of biomedical applications, the first important feature is the biocompatibility of the new advanced material. In this chapter, the general biocompatibility concept, test systems to determine biocompatibility, examples of bioinspired materials and their altered biocompatibility and future expectations from these novel bioinspired materials will be discussed.