In this review paper, the definition of the tissue engineering (TE) was comprehensively explored towards scaffold fabrication techniques and applications. Scaffold properties and features in TE, biological aspects, scaffold material composition, scaffold structural requirements, and old and current manufacturing technologies were reported and discussed. In almost all the reviewed reports, the TE definition denotes renewal, development, and repairs of damaged tissues caused by various factors such as disease, injury, or congenital disabilities. TE is multidisciplinary that combines biology, biochemistry, clinical medicine, and materials science whose application in cellular systems such as organ transplantation serves as a delivery vehicle for cells and drug. According to the previous literature and this review, the scaffold fabrication techniques can be classified into two main categories: conventional and modern techniques. These TE fabrication techniques are applied in the scaffold building which later on are used in tissue and organ structure. The benefits and drawbacks of each of the fabrication techniques have been described in conjunction with current areas of research devoted to deal with some of the challenges. To figure out, the highlighted aspects aimed to define the advancements and challenges that should be addressed in the scaffold design for tissue engineering. Additionally, this study provides an excellent review of original numerical approaches focused on mechanical characteristics that can be helpful in the scaffold design assessment in the analysis of scaffold parameters in tissue engineering.

Grafting is a substitute technique to boost tolerance against abiotic stresses, which also diminish root syndromes from soil-borne pathogens and increase crop production. This piloted study evaluates the tongue grafting technique and investigates the influence of grafting hybrid cucumber (Kalam F1) scion onto local cucurbitaceous and hybrid rootstocks on plant morphology, fruit yield, nutrients concentration in different plant organs, and postharvest fruit quality during three seasons of 2017 to 2019, consecutively. The research design was completely randomized (CRD), and the effects of grafted rootstocks were determined with comparison of percent plant survival, growth characteristics, fruit yield, and texture properties (during storage). The experimental results indicated that all rootstocks presented compatibility with hybrid cucumber (Kalam F1) scion and showed significant comparable performance with hybrid rootstock during all seasons in terms of vegetative growth, yield, and storage except pumpkin (68% during the 1st, 74% during the 2nd, and 71% during the 3rd seasons). The plants with Bottle gourd (Lagrenaria siceraria) rootstock showed the highest survival rate as compared to all other cucurbitaceous and hybrid rootstocks during three seasons: 94%, 98%, and 93%, respectively. Similarly, the other factors such as plant height (613, 622, and 615 cm), fruit weight (282, 281, and 277 g), fruit shape index (7.77, 8.18, and 8.25), Total soluble solids (TSS) (5.1%, 5.17%, and 4.96%), fruit dry matter (4.55%, 4.625, and 4.57%), fruit yield (4.37, 4.9, and 3.95 kg/plant), and disease resistance index (<3) during three seasons were comparable with all other cucurbitaceous and hybrid rootstocks. The lower survival rate of pumpkin rootstock made a significant poor performance with lower growth, yield, nutrients, and storage than all other cucurbitaceous rootstocks. Ridge and Bitter gourds showed improved results for vegetative growth, yield, fruit characteristics, nutritional values, and storage performance than that of hybrid cucumber rootstock. The fruit textural and physical properties were found significantly better at 10 °C than 25 °C for 10 days of storage without quality deterioration, while no impact was perceived on physical properties of the fruit. According to economic analysis, the grafted cucumber production system showed higher net return and benefit-cost ratio (BCR) in comparison with the real rooted hybrid cucumber production system, which was assumed to be profitable practice.

The density of herbaceous crops creates a suitable environment to produce pathogens in the soil that intensify the attack of pathogens traditionally controlled by disinfectant, which are mostly prohibited and unlisted because of their toxicity. Grafting is an alternative technique to enhance abiotic stress tolerance and reduce root diseases due to soil-borne pathogens, thus enhancing crop production. This research study was conducted during the crop season of 2017 and 2018 in order to investigate the interactive effect of different grafting techniques of hybrid scion onto local rootstocks on plants survival, plant phenological growth, fruit yield and fruit quality under a controlled environment. The hybrid cucumber was also planted self-rooted. The cucumber (Cucumis sativus L.) cv. Kalaam F1, Syngenta was grafted onto four local cucurbitaceous rootstocks; ridge gourd (Luffa operculate Cogn.), bitter gourd (Momordica charantia L.), pumpkin (Cucurbita pepo L.), bottle gourd (Lagenaria siceraria (Molina) Standl.) using splice grafting, tongue approach, single cotyledon and hole insertion grafting techniques and self-rooted hybrid cucumber under greenhouse conditions. The experimental results indicated that all local cucurbitaceous rootstocks showed a high compatibility with hybrid cucumber scion in the splice grafting method compared to other grafting and non-grafted methods. Lagenaria siceraria rootstocks were found highly compatible with cucumber cv Kalaam scion which gave significantly maximum plant survival rates (95%) due to high sap contents, high SPAD value, better vegetative growth and maximum fruit yield when compared with other rootstocks by employing the splice grafting method followed by tongue approach, single cotyledon and hole insertion grafting while the fruit quality of all rootstocks was observed to be similar. The non-grafted cucumber cv. Kalaam F1 showed significant results of plant vegetative growth, fruit development and fruit quality and encountered grafting methods while the lowest result were associated with the hole insertion grafting method in all scion/rootstock combinations. The grafted plants have no significant effect on cucumber fruit dry matter and fruit quality while the fruit mineral compositions (N, P, K, Ca and Mg) were higher among grafted and non-grafted plant fruits. The results indicate that grafting hybrid cucumber onto four local cucurbitaceous rootstocks influenced growth, yield and fruit quality. Grafting can be alternative and control measure for soil-borne disease and to enhance cucumber production.

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