Light sheet microscopy (LSM) is an evolving optical imaging technique with a plane illumination for optical sectioning and volumetric imaging spanning cell biology, embryology, and in vivo live imaging. Here, we focus on emerging biomedical applications of LSM for tissue samples. Decoupling of the light sheet illumination from detection enables high-speed and large field-of-view imaging with minimal photobleaching and phototoxicity. These unique characteristics of the LSM technique can be easily adapted and potentially replace conventional histopathological procedures. In this review, we cover LSM technology from its inception to its most advanced technology; in particular, we highlight the human histopathological imaging applications to demonstrate LSM's rapid diagnostic ability in comparison with conventional histopathological procedures. We anticipate that the LSM technique can become a useful three-dimensional imaging tool for assessing human biopsies in the near future.

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International students are highly vulnerable to the risk of mental health worsening before and during the pandemic . This study investigated international students' mental health pre-and post-pandemic (COVID-19). Methods: It is a longitudinal study, and data were collected online, pre-pandemic (N = 470) and during the pandemic (N = 420). Using a random sampling technique, a self-administered questionnaire was used to measure mental health, including depression and anxiety. Results: Findings show that international students' mental health was good in pre-pandemic. Meanwhile, international students were found to be more depressed and anxious during the pandemic. Findings also investigated that in the pre-pandemic phase, young students' and mainly females' mental health was worsened. Conclusion:This study concluded that students' mental problems are alarming, so the university should provide psychological services for the student's mental health. Post-pandemic is leaving long-lasting psychological effects and will require further investigation.

In the presence of noise in normal dispersion fiber mode-locked lasers, "dissipative resonance" disappears, which leads to instability of spectral broadening and makes the asymptotic accumulation of energy impossible. We experimentally generate dissipative soliton molecules-like spectral peaks and rogue waves in the frequency domain in a self-mode-locked fiber laser. Due to noise in the initial triangular-shaped mode locked wave, these molecules are arranged in the form of lobes on the longer wavelength side, thus forming three virtual groups of soliton molecules. Further dissipative accumulation of power in the mode-locked wave ultimately leads to the formation of a blue-shifted group of three rogue waves, due to the effective fusion of adjacent soliton molecules. This is the first report of an experimental observation of blue-shifted and three rogue waves in a single experiment. The results have far reaching implications for experimentation with soliton collisions, optical rogue waves, and understanding of hydrodynamics.Index Terms-Passive mode-lock fiber lasers, normal dispersion, instability of dissipative soliton resonance, bound solitons, supercontinuum, rogue waves.

We describe the prototype of a focal plane imager for the characterization of fabrication errors in diffractive optics. We also demonstrate the use of a high dynamic range imaging method to identify small errors in a hologram and a grating that can be traced to the fabrication method.