This study was carried out to separate acute myeloid leukemia (AML) blast cells and studies their proliferation in short-term culture. The separation procedure include three steps; Ficoll gradient separation, depletion of macrophages and depletion of (lymphocytes and of monocytes) for preparation of highly pure native AML blast cells from blood samples collected from patients with moderate blast percentage. Results showed that this procedure is an inefficient due to a decrease in total cell number and contamination with other cells after each separation step. Proliferation of native AML blast cells in short term-culture by cultivating isolated AML blast cells in RPMI medium supplemented with 20% human plasma at a concentration 1×106/ml in the presence and absence of colony -stimulating factor which was provided by conditioned media (PHA-leucocytes-, plasmacytoma cell line- and Hep-2 conditioned medium. The effect of each conditioned medium on proliferation of AML blast cells was studied separately. Results showed that plasmacytoma cell line conditioned medium didnot stimulate the proliferation of native AML blast cells, while cells seeded on media containing 10%PHA-LCM showed an increase in cell number and growth of the cells was observed for approximately 3 days and then decreased.

Background: To assess the awareness of Covid-19 among the Physiotherapists of Sindh, Pakistan, in order to check the level of knowledge present regarding the pandemics among the working physiotherapist of Pakistan. Material & Methods: A cross sectional study was carried out in different hospitals of Sindh, Pakistan from June to July 2020. A self-constructed questionnaire by Pursuing the clinical and community administration of COVID-19 given by the National Health Commission of the People's Republic of China was used among the 343 Physiotherapists and SPSS version 23 was used to analyze the data. Results: The majority (n=207, 60.3%) were male, having age between 33 and above years (n=227, 66.2%) and (n=304, 88.6%) were married. In educational status (n=230, 67.1%) were postgraduate and (n=304, 88.6%) belongs to urban area. During the assessment of awareness towards COVID-19 results showed that majority (n=321, 93.6%) were aware of COVID-19. Conclusion: This Study concluded that the physiotherapists who are working in different hospitals of Sindh, Pakistan are well aware of COVID-19.The awareness can be due to their self-interest in infection preventive measure and quality of education being provided by their respective institutions.